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KIAA0930 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0930 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA0930 antibodies →
$504.00each
Catalog Number: LS-G113324-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C22orf9(KIAA0930) (NM_015264), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C22orf9 | KIAA0930 | BK268H5.C22.1
Origin Species
Human
NCBI GenBank
AA029335

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSQAAAEWR NWASWEVSSS LSGCSMGCFK DDRIVFWTWM FSTYFMEKWA PRQDDMLFYV
61RRKLAYSGSE SGADGRKAAE PEVEVEVYRR DSKKLPGLGD PDIDWEESVC LNLILQKLDY
121MVTCAVCTRA DGGDIHIHKK KSQQVFASPS KHPMDSKGEE SKISYPNIFF MIDSFEEVFS
181DMTVGEGEMV CVELVASDKT NTFQGVIFQG SIRYEALKKV YDNRVSVAAR MAQKMSFGFY
241KYSNMEFVRM KGPQGKGHAE MAVSRVSTGD TSPCGTEEDS SPASPMHERV TSFSTPPTPE
301RNNRPAFFSP SLKRKVPRNR IAEMKKSHSA NDSEEFFRED DGGADLHNAT NLRSRSLSGT
361GRSLVGSWLK LNRADGNFLL YAHLTYVTLP LHRILTDILE VRQKPILMT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

409 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.