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KIAA0922 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0922 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA0922 antibodies →
$725.00each
Catalog Number: LS-G113387-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM131L(KIAA0922) (NM_015196), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0922 | Transmembrane protein 131-like | TMEM131L
Origin Species
Human
NCBI GenBank
NM_015196
SwissProt
A2VDJ0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
163.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKTSFRIIF LPTEEGSIES SLFINTSSYG VLSYHVSGIG TRRISTEGSA KQLPNAYFLL
61PKVQSIQLSQ MQAETTNTSL LQVQLECSLH NKVCQQLKGC YLESDDVLRL QMSIMVTMEN
121FSKEFEENTQ HLLDHLSIVY VATDESETSD DSAVNMYILH SGNSLIWIQD IRHFSQRDAL
181SLQFEPVLLP TSTTNFTKIA SFTCKAATSC DSGIIEDVKK TTHTPTLKAC LFSSVAQGYF
241RMDSSATQFH IETHENTSGL WSIWYRNHFD RSVVLNDVFL SKETKHMLKI LNFTGPLFLP
301PGCWNIFSLK LAVKDIAINL FTNVFLTTNI GAIFAIPLQI YSAPTKEGSL GFEVIAHCGM
361HYFMGKSKAG NPNWNGSLSL DQSTWNVDSE LANKLYERWK KYKNGDVCKR NVLGTTRFAH
421LKKSKESESF VFFLPRLIAE PGLMLNFSAT ALRSRMIKYF VVQNPSSWPV SLQLLPLSLY
481PKPEALVHLL HRWFGTDMQM INFTTGEFQL TEACPYLGTH SEESRFGILH LHLQPLEMKR
541VGVVFTPADY GKVTSLILIR NNLTVIDMIG VEGFGARELL KVGGRLPGAG GSLRFKVPES
601TLMDCRRQLK DSKQILSITK NFKVENIGPL PITVSSLKIN GYNCQGYGFE VLDCHQFSLD
661PNTSRDISIV FTPDFTSSWV IRDLSLVTAA DLEFRFTLNV TLPHHLLPLC ADVVPGPSWE
721ESFWRLTVFF VSLSLLGVIL IAFQQAQYIL MEFMKTRQRQ NASSSSQQNN GPMDVISPHS
781YKSNCKNFLD TYGPSDKGRG KNCLPVNTPQ SRIQNAAKRS PATYGHSQKK HKCSVYYSKH
841KTSTAAASST STTTEEKQTS PLGSSLPAAK EDICTDAMRE NWISLRYASG INVNLQKNLT
901LPKNLLNKEE NTLKNTIVFS NPSSECSMKE GIQTCMFPKE TDIKTSENTA EFKERELCPL
961KTSKKLPENH LPRNSPQYHQ PDLPEISRKN NGNNQQVPVK NEVDHCENLK KVDTKPSSEK
1021KIHKTSREDM FSEKQDIPFV EQEDPYRKKK LQEKREGNLQ NLNWSKSRTC RKNKKRGVAP
1081VSRPPEQSDL KLVCSDFERS ELSSDINVRS WCIQESTREV CKADAEIASS LPAAQREAEG
1141YYQKPEKKCV DKFCSDSSSD CGSSSGSVRA SRGSWGSWSS TSSSDGDKKP MVDAQHFLPA
1201GDSVSQNDFP SEAPISLNLS HNICNPMTVN SLPQYAEPSC PSLPAGPTGV EEDKGLYSPG
1261DLWPTPPVCV TSSLNCTLEN GVPCVIQESA PVHNSFIDWS ATCEGQFSSA YCPLELNDYN
1321AFPEENMNYA NGFPCPADVQ TDFIDHNSQS TWNTPPNMPA AWGHASFISS PPYLTSTRSL
1381SPMSGLFGSI WAPQSDVYEN CCPINPTTEH STHMENQAVV CKEYYPGFNP FRAYMNLDIW
1441TTTANRNANF PLSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1454 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.