Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0828 / AHCYL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125109-100
Catalog Number: LS-G125109
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AHCYL2 (NM_001130720), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AdoHcyase 3 | AHCYL2 | Adenosylhomocysteinase-like 2 | ADOHCYASE3 | KIAA0828
- Origin Species
- Human
- NCBI GenBank
- NM_015328
- SwissProt
- Q96HN2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVQVVSAAA AAKVPEVELK DLSPSEAESQ LGLSTAAVGA MAPPAGGGDP EAPAPAAERP61PVPGPGSGPA AALSPAAGKV PQASAMKRSD PHHQHQRHRD GGEALVSPDG TVTEAPRTVK121KIQFADQKQE FNKRPTKIGR RSLSRSISQS STDSYSSAAS YTDSSDDETS PRDKQQKNSK181GSSDFCVKNI KQAEFGRREI EIAEQEMPAL MALRKRAQGE KPLAGAKIVG CTHITAQTAV241LMETLGALGA QCRWAACNIY STLNEVAAAL AESGFPVFAW KGESEDDFWW CIDRCVNVEG301WQPNMILDDG GDLTHWIYKK YPNMFKKIKG IVEESVTGVH RLYQLSKAGK LCVPAMNVND361SVTKQKFDNL YCCRESILDG LKRTTDMMFG GKQVVVCGYG EVGKGCCAAL KAMGSIVYVT421EIDPICALQA CMDGFRLVKL NEVIRQVDIV ITCTGNKNVV TREHLDRMKN SCIVCNMGHS481NTEIDVASLR TPELTWERVR SQVDHVIWPD GKRIVLLAEG RLLNLSCSTV PTFVLSITAT541TQALALIELY NAPEGRYKQD VYLLPKKMDE YVASLHLPTF DAHLTELTDE QAKYLGLNKN601GPFKPNYYRYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
610 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.