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KIAA0556 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0556 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA0556 antibodies →
$725.00each
Catalog Number: LS-G113392-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0556 (NM_015202), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0556
Origin Species
Human
NCBI GenBank
AB011128
SwissProt
O60303

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
180.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGQTLRKAE RSWSCSREKK EGYAKDMVTD FDEKHDEYLI LLQQRNRILK HLKSKDPVQL
61RLEHLEQGFS VYVNGANSEL KSSPRKAIHS DFSRSASHTE GTHDYGRRTL FREAEEALRR
121SSRTAPSKVQ RRGWHQKSVQ IRTEAGPRLH IEPPVDYSDD FELCGDVTLQ ANNTSEDRPQ
181ELRRSLELSV NLQRKQKDCS SDEYDSIEED ILSEPEPEDP ALVGHPRHDR PPSSGDWTQK
241DVHGEQETEG RSSPGPDTLV VLEFNPASKS HKRERNLSAK RKDNAEVFVP TKPEPNLTPQ
301APAVFPDQER MCSRPGSRRE RPLSATRKTL CEAEYPEEDA SAVLQAIQVE NAALQRALLS
361RKAEQPASPL QDAEGPPAKP WTSLLEEKEE TLELLPITTA TTTQEPAGAA GGARAINQAM
421DRIGLLGSRQ QQKLLKVLQA VESDSAHLGR VVSPTKEQVS DTEDKQRMRA DEIKDAIYVT
481MEILSNWGNS WWVGLTEVEF FDLNDTKLYV SPHDVDIRNT ATPGELGRLV NRNLAGKKDS
541SPWTCPFHPP LQLFFVIRNT RQLGDFHLAK IKVRNYWTAD GDLDIGAKNV KLYVNRNLIF
601NGKLDKGDRE APADHSILVD QKNEKSEQLE EAMNAHSEES KGTHEMAGAS GDKELGLGCS
661PPAETLADAK LSSQGNVSGK RKNSTNCRKD SLSQLEEYLR LSAVPTSMGD MPSAPATSPP
721VKCPPVHEEP SLIQQLENLM GRKICEPPGK TPSWLQPSPT GKDRKQGGRK PKPLWLSPEK
781PLAWKGRLPS DDVIGEGPGE TEARDKGLRH EPGWGTSRSV NTKERPQRAT TKVHSDDSDI
841FNQPPNRERP ASGRRGSRKD AGSSSHGDDQ PASREDTWSS RTPSRSRWRS EQEHTLHESW
901SSLSAFDRSH RGRISNTELP GDILDELLQQ KSSRHSDLPP SKKGEQPGLS RGQDGYSGET
961DAGGDFKIPV LPYGQRLVID IKSTWGDRHY VGLNGIEIFS SKGEPVQISN IKADPPDINI
1021LPAYGKDPRV VTNLIDGVNR TQDDMHVWLA PFTRGRSHSI TIDFTHPCHV ALIRIWNYNK
1081SRIHSFRGVK DITMLLDTQC IFEGEIAKAS GTLAGAPEHF GDTILFTTDD DILEAIFYSD
1141EMFDLDVGSL DSLQDEEAMR RPSTADGEGD ERPFTQAGLG ADERIPELEL PSSSPVPQVT
1201TPEPGIYHGI CLQLNFTASW GDLHYLGLTG LEVVGKEGQA LPIHLHQISA SPRDLNELPE
1261YSDDSRTLDK LIDGTNITME DEHMWLIPFS PGLDHVVTIR LDRAESIAGL RFWNYNKSPE
1321DTYRGAKIVH VSLDGLCVSP PEGFLIRKGP GNCHFDFAQE ILFVDYLRAQ LLPQPARRLD
1381MRSLECASMD YEAPLMPCGF IFQFQLLTSW GDPYYIGLTG LELYDERGEK IPLSENNIAA
1441FPDSVNSLEG VGGDVRTPDK LIDQVNDTSD GRHMWLAPIL PGLVNRVYVI FDLPTTVSMI
1501KLWNYAKTPH RGVKEFGLLV DDLLVYNGIL AMVSHLVGGI LPTCEPTVPY HTILFTEDRD
1561IRHQEKHTTI SNQAEDQDVQ MMNENQIITN AKRKQSVVDP ALRPKTCISE KETRRRRC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1618 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.