Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0467 / SZT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113340-100
Catalog Number: LS-G113340
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SZT2 (NM_015284), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf84 | Protein SZT2 | RP11-506B15.1 | RP11-506B15.3 | SZT2B | KIAA0467 | SZT2 | SZT2A
- Origin Species
- Human
- NCBI GenBank
- AK027078
- SwissProt
- Q5T011
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 371.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASERPEPEV EEAGQVFLLM KKDYRISRNV RLAWFLSHLH QTVQATPQEM LLQSEQELEV61LSVLPPGWQP DEPVVPRPFL LVPSTRVTFL AWQYRFVIEL DLSPSTGIVD DSTGEILFDE121VFHALSRCLG GLLRPFRVPG SCIDFQPEIY VTIQAYSSII GLQSHQVLVQ GCLLDPSQRE181VFLQQIYEQL CLFEDKVATM LQQQYDPQSQ AEDQSPDSGD LLGRKVGVSM VTADLGLVSM241IRQGILALQL LPSNSSAGII VITDGVTSVP DVAVCETLLN QLRSGTVACS FVQVGGVYSY301DCSFGHVPNV ELMKFIAMAT FGSYLSTCPE PEPGNLGLTV YHRAFLLYSF LRSGEALNPE361YYCGSQHRLF NEHLVSASSN PALALRRKKH TEKEVPADLV STVSVRLREG YSVREVTLAK421GGSQLEVKLV LLWKHNMRIE YVAMAPWPLE PEGPRVTRVE VTMEGGYDIL HDVSCALRQP481IRSLYRTHVI RRFWNTLQSI NQTDQMLAHL QSFSSVPEHF TLPDSTKSGV PLFYIPPGST541TPVLSLQPSG SDSSHAQFAA YWKPVLSMDA NSWQRWLHMH RLVLILEHDT PIPKHLHTPG601SNGRYSTIQC RISHSSLTSL LRDWSSFVLV EGYSYVKLLS SAPDQPPNSF YMVRIISKAP661CMVLRLGFPI GTPAPARHKI VSGLREEILR LRFPHRVQSK EPTPKVKRKG LGGAGGGSSP721SKSPPVLGPQ QALSDRPCLV VLHKPLDKLL IRYEKLPLDY RAPFLLTLEP PGPLPLVSGR781SASSSLASLS RYLYHQRWLW SVPSGLAPAL PLSAIAQLLS ILTEVRLSEG FHFACSGEGI841INMVLELPIQ NEPPGQAAAE EKHTCVVQYI LFPPHSTSTK DSFSTDDDND VEVEALEGDS901ELNLVTEVWV EPQYGRVGPG PGIWKHLQDL TYSEIPQALH PRDAACIGSM LSFEYLIQLC961QSKEWGPLPP EPRVSDGLDQ GGDTCVHEIP FHFDLMGLLP QCQQLQMFFL LLARGAEGPL1021LGVHGIPKEQ AVGSTQATGD SAFTSLSVGL PETLKPLISA QPPQWRCYAR LVNPQHVFLT1081FLPATFSDVQ RLAACGLEGP PQEETKPKFG DWSGAPSLKD LGGTGIKATK SHVPVLSVTL1141ASDNAQNQGE LSPPFRRDLQ AYAGRQASQT ESADGPRTRC PVYIYSCSLE ALREQMVGMQ1201PPQAPRDLIF RTQFLDHPSP SSAWMEPRYK EAANHCALLQ EHAQRCYVRG LFRSLQQAQS1261VTSQDLLTAV DACEELLQEI DITPFLLALC GHTWGLPHAP PSPGPLSPGP FSSSMEEGAE1321PRERAILASE SSIETEDLSE PEFQSTRVPG IPDPGPEISL TDVCQLRGEA HGALHSVIQE1381KFLEISRLHF RTVPSNPHYF FYCPPSSRRE DEGPRDTVDR KISDLEFSEA ELMGEEGDTS1441ACCVVTESDP ELEVEYRESR ESDLGPAGLD SASLSDVDTV NPDEDSFSIL GGDSPTGPES1501FLHDLPPLFL HLTCSVRLRG QHSSVPVCSL PTCLGQVLSS LEGPPVGGRV PLRDLSVTLD1561VFMLTLPLEV ELPTASDPQH HRSTSESSAS FPRSPGQPSS LRSDDGLGPP LPPPEEERHP1621GLSNLATPHR LAIETTMNEI RWLLEDEMVG ALRRGGIPQS PALHRAAAHI HSSPGRSTCL1681RQTLPLSFVF GPERSLTQFK EEFRRLHLPG HVLLEDPDSG FFFVAAGQQP GGSHGEPSSA1741AWAWHSHEDR AEGIEGETLT ASPQAPGSPE DSEGVPLISL PRVPQGGSQP GPSRGLSLMS1801SQGSVDSDHL GYDGGSSGSD SEGPNDTLGE KAPFTLRTPP GPAPPQPSLS GLPGPCLPDF1861WLIVRVLQDR VEVYAHARSL IREDGGPGTE CRHLQQLLVR RVGEICREVN QRLLLQDLHD1921SHVCNSLLVA ESEEDLWRSE TPFHSRQRAP LPSDDYAADE SCAPRGYLAA TMQFVPGHFS1981CDVVWGTVIR VHSRLKMGPS MGVSRAIQAL RSVLNAFSVV NRKNMFVYQE RATKAVYYLR2041LLETSCSDRP WKGDALPPSL ALSRSQEPIY SEEASGPRSP LDMVSSRSSD AARPVGQVDR2101HIQLLVHGVG QAGPEITDEL VRVLCRRLDE ATLDVITVML VRNCKLTPAD VEFIQPPGSL2161PSEVLHLALP TSCRPWLPAL AWYLRQNLLI FLHSPKYTDS NSRNHFQHPL PPQGGLPDLD2221IYLYNKPGGQ GTGGKGVACI TLAFVDEGGA PLSLALWPPS SPGPPDPLRE EEFEQLTQVI2281RCPVVVDSSS AQNGAPRLRL DVWEKGNISI VQLEEKLRGA ARQALADAII ELQLLPASLC2341TEDTPTGSLR NGSLETKSSA GRASTFPPAP VPGEPVTPPS KAGRRSFWDM LSKTECGDLG2401SPKTTDDIVL DRPEDTRGRR RHKTESVRTP GGAERAPGSD SGAQRQKRRT TQLEEGEVGT2461LHPVFARVAQ RWMEFMVQIG CASVSRSSAH MVSRFLLPSI LSEFTALVTS MAGDTSVRIF2521EQHLGSEPEI FGPCSPGQLG PSPRPAAERH LLLLGRNFLQ WRRPTQQAAK AMQRFEPGGD2581GSSGRNAPRQ RLLLLEVVDK KLQLLTYNWA PDLGAALGRA LVRLVQWQNA RAHLIFCLLS2641QKLGLFHHYG QLDFPVRDEK EPNPFLLPTM EVETLIRSAS PPLSREQGRL SGSSRGGGPL2701PLDTFPFDEA LRDITAARPS SVLGPVPRPP DPVTYHGQQF LEIKMAERRE LERQMKMENL2761FVTWQQRSTP ATMPISAGEL ETLKQSSRLV HYCATAMLFD PAAWLHGPPE TSGPPDGQRR2821HRPESGSGSR EAPTSCESLD VSPPGAREEP WLKELSLAFL QQYVQYLQSI GFVLVPLRPP2881SPARSTSRPR AMAILGTEGR GSFSCPKTKT DGSPKSTSSP VTTYHLQRAL PGGIILMELA2941FQGCYFCVKQ FALECSRIPM GQAVNSQLSM LFTEECDKVR DLMHVHSFSY DFHLRLVHQH3001VLGAHLVLRH GYHLTTFLRH FLAHHPDGPH FGRNHIYQGT LELPTPLIAA HQLYNYVADH3061ASSYHMKPLR MARPGGPEHN EYALVSAWHS SGSYLDSEGL RHQDDFDVSL LVCHCAAPFE3121EQGEAERHVL RLQFFVVLTS QRELFPRLTA DMRRFRKPPR LPPEPEAPGS SAGSPGEASG3181LILAPGPAPL FPPLAAEVGM ARARLAQLVR LAGGHCRRDT LWKRLFLLEP PGPDRLRLGG3241RLALAELEEL LEAVHAKSIG DIDPQLDCFL SMTVSWYQSL IKVLLSRFPQ SCRHFQSPDL3301GTQYLVVLNQ KFTDCFVLVF LDSHLGKTSL TVVFREPFPV QPQDSESPPA QLVSTYHHLE3361SVINTACFTL WTRLLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
3375 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.