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KIAA0408 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0408 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA0408 antibodies →
$504.00each
Catalog Number: LS-G113746-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0408 (NM_014702), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0408 | RP3-403A15.2
Origin Species
Human
NCBI GenBank
AB007868

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLHKQWENT ETNWHKEKME LLDQFDNERK EWESQWKIMQ KKIEELCREV KLWRKININE
61RAKIIDLYHE KTIPEKVIES SPNYPDLGQS EFIRTNHKDG LRKENKREQS LVSGGNQMCK
121EQKATKKSKV GFLDPLATDN QKECEAWPDL RTSEEDSKSC SGALSTALEE LAKVSEELCS
181FQEEIRKRSN HRRMKSDSFL QEMPNVTNIP HGDPMINNDQ CILPISLEKE KQKNRKNLSC
241TNVLQSNSTK KCGIDTIDLK RNETPPVPPP RSTSRNFPSS DSEQAYERWK ERLDHNSWVP
301HEGRSKRNYN PHFPLRQQEM SMLYPNEGKT SKDGIIFSSL VPEVKIDSKP PSNEDVGLSM
361WSCDIGIGAK RSPSTSWFQK TCSTPSNPKY EMVIPDHPAK SHPDLHVSND CSSSVAESSS
421PLRNFSCGFE RTTRNEKLAA KTDEFNRTVF RTDRNCQAIQ QNHSCSKSSE DLKPCDTSST
481HTGSISQSND VSGIWKTNAH MPVPMENVPD NPTKKSTTGL VRQMQGHLSP RSYRNMLHEH
541DWRPSNLSGR PRSADPRSNY GVVEKLLKTY ETATESALQN SKCFQDNWTK CNSDVSGGAT
601LSQHLEMLQM EQQFQQKTAV WGGQEVKQGI DPKKITEESM SVNASHGKGF SRPARPANRR
661LPSRWASRSP SAPPALRRTT HNYTISLRSE ALMV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

694 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.