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KIAA0391 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0391 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA0391 antibodies →
$504.00each
Catalog Number: LS-G113792-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MRPP3(KIAA0391) (NM_014672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0391 | MRPP3
Origin Species
Human
NCBI GenBank
AB002389
SwissProt
O15091

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTFYLFGIRS FPKLWKSPYL GLGPGHSYVS LFLADRCGIR NQQRLFSLKT MSPQNTKATN
61LIAKARYLRK DEGSNKQVYS VPHFFLAGAA KERSQMNSQT EDHALAPVRN TIQLPTQPLN
121SEEWDKLKED LKENTGKTSF ESWIISQMAG CHSSIDVAKS LLAWVAAKNN GIVSYDLLVK
181YLYLCVFHMQ TSEVIDVFEI MKARYKTLEP RGYSLLIRGL IHSDRWREAL LLLEDIKKVI
241TPSKKNYNDC IQGALLHQDV NTAWNLYQEL LGHDIVPMLE TLKAFFDFGK DIKDDNYSNK
301LLDILSYLRN NQLYPGESFA HSIKTWFESV PGKQWKGQFT TVRKSGQCSG CGKTIESIQL
361SPEEYECLKG KIMRDVIDGG DQYRKTTPQE LKRFENFIKS RPPFDVVIDG LNVAKMFPKV
421RESQLLLNVV SQLAKRNLRL LVLGRKHMLR RSSQWSRDEM EEVQKQASCF FADDISEDDP
481FLLYATLHSG NHCRFITRDL MRDHKACLPD AKTQRLFFKW QQGHQLAIVN RFPGSKLTFQ
541RILSYDTVVQ TTGDSWHIPY DEDLVERCSC EVPTKWLCLH QKT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

583 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.