Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0368 / ECM29 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120186-100
Catalog Number: LS-G120186
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ECM29(KIAA0368) (NM_001080398), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ECM29 | KIAA0368 | RP11-386D8.2 | Homolog of yeast Ecm29
- Origin Species
- Human
- NCBI GenBank
- AB002366
- SwissProt
- Q5VYK3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 223.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSRTKRRQK VKISISKINH SSYRKEHRPF WEHQPPPHQN WAVGGRCGAG ARPGLSQRRG61REVRREAQGG ASSRRLRARL YFSESTSLPL DRRGSPGPLG PAPHGLRRAA PRGPARRTPR121STALTSRRRA QRPRHGDRLR LRSEARPRAA GDWPCARGGA VRPGRPHRPP RVESARGAHA181GRAATDQLER VFLRLGHAET DEQLQNIISK FLPPVLLKLS STQEGVRKKV MELLVHLNKR241IKSRPKIQLP VETLLVQYQD PAAVSFVTNF TIIYVKMGYP RLPVEKQCEL APTLLTAMEG301KPQPQQDSLM HLLIPTLFHM KYPVESSKSA SPFNLAEKPK TVQLLLDFML DVLLMPYGYV361LNESQSRQNS SSAQGSSSNS GGGSGIPQPP PGMSFYAAKR VIGDNPWTPE QLEQCKLGIV421KFIEAEQVPE LEAVLHLVIA SSDTRHSVAT AADLELKSKQ SLIDWNNPAI INKMYKVYLG481DIPLKTKEGA VLKPELKRDP VSTRVKLKIV PHLLRSRQAA ETFPANIQVV YDGLFGTNTN541SKLRTLSLQF VHHICITCPE IKIKPLGPML LNGLTKLINE YKEDPKLLSM AYSAVGKLSS601RMPHLFTKDI ALVQQLFEAL CKEEPETRLA IQEALSMMVG AYSTLEGAQR TLMEALVASY661LIKPEVQVRQ VAVKFASTVF PSDHIPSRYL LLLAAGDPRE EVHGEAQRVL RCLPGRNRKE721STSEQMPSFP EMVYYIQEKA SHRMKTPVKY MTGTTVLPFN PAAFGEIVLY LRMCLAHSAG781VVPTSQSLAD MQDHAPAIGR YIRTLMSSGQ MAPSSSNKSG ETNPVQIYIG LLQQLLAGVG841GLPVMYCLLE AVSVYPEKLA TKFVDKTEWI KSLMNNSKEE MRELAALFYS VVVSTVSGNE901LKSMIEQLIK TTKDNHSPEI QHGSLLALGF TVGRYLAKKK MRMSEQQDLE RNADTLPDQE961ELIQSATETI GSFLDSTSPL LAIAACTALG EIGRNGPLPI PSEGSGFTKL HLVESLLSRI1021PSSKETNKMK ERAIQTLGYF PVGDGDFPHQ KLLLQGLMDS VEAKQIELQF TIGEAITSAA1081IGTSSVAARD AWQMTEEEYT PPAGAKVNDV VPWVLDVILN KHIISPNPHV RQAACIWLLS1141LVRKLSTHKE VKSHLKEIQS AFVSVLSEND ELSQDVASKG LGLVYELGNE QDQQELVSTL1201VETLMTGKRV KHEVSGETVV FQGGALGKTP DGQGLSTYKE LCSLASDLSQ PDLVYKFMNL1261ANHHAMWNSR KGAAFGFNVI ATRAGEQLAP FLPQLVPRLY RYQFDPNLGI RQAMTSIWNA1321LVTDKSMVDK YLKEILQDLV KNLTSNMWRV RESSCLALND LLRGRPLDDI IDKLPEIWET1381LFRVQDDIKE SVRKAAELAL KTLSKVCVKM CDPAKGAAGQ RTIAALLPCL LDKGMMSTVT1441EVRALSINTL VKISKSAGAM LKPHAPKLIP ALLESLSVLE PQVLNYLSLR ATEQEKAAMD1501SARLSAAKSS PMMETINMCL QYLDVSVLGE LVPRLCELIR SGVGLGTKGG CASVIVSLTT1561QCPQDLTPYS GKLMSALLSG LTDRNSVIQK SCAFAMGHLV RTSRDSSTEK LLQKLNGWYM1621EKEEPIYKTS CALTIHAIGR YSPDVLKNHA KEVLPLAFLG MHEIADEEKS EKEECNLWTE1681VWQENVPGSF GGIRLYLQEL ITITQKALQS QSWKMKAQGA IAMASIAKQT SSLVPPYLGM1741ILTALLQGLA GRTWAGKEEL LKAIACVVTA CSAELEKSVP NQPSTNEILQ AVLKECSKEN1801VKYKIVAISC AADILKATKE DRFQEFSNIV IPLIKKNSLE SSGVRTTKNE EENEKEKELQ1861LEYLLGAFES LGKAWPRNAE TQRCYRQELC KLMCERLKLS TWKVQLGVLQ SMNAFFQGLM1921LLEEEHADPE ALAEILLETC KSITYSLENK TYSSVRTEAL SVIELLLKKL EESKQWECLT1981SECRVLLIES LATMEPDSRP ELQEKAALLK KTLENLESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
2017 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.