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KIAA0355 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0355 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA0355 antibodies →
$504.00each
Catalog Number: LS-G113770-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0355 (NM_014686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_014686
SwissProt
O15063

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYCCSAQDSK MDYKRRFLLG GSKQKVQQHQ QYPMPELGRA LSAPLASTAT TAPLGSLTAA
61GSCHHAMPHT TPIADIQQGI SKYLDALNVF CRASTFLTDL FSTVFRNSHY SKAATQLKDV
121QEHVMEAASR LTSAIKPEIA KMLMELSAGA ANFTDQKEFS LQDIEVLGRC FLTVVQVHFQ
181FLTHALQKVQ PVAHSCFAEV IVPEKKNSGS GGGLSGMGHT PEVEEAVRSW RGAAEATSRL
241RERGCDGCLA GIEVQQLFCS QSAAIPEHQL KELNIKIDSA LQAYKIALES LGHCEYAMKA
301GFHLNPKAIE ASLQGCCSEA EAQQTGRRQT PPQPMQCELP TVPVQIGSHF LKGVSFNESA
361ADNLKLKTHT MLQLMKEAGC YNGITSRDDF PVTEVLNQVC PSTWRGACKT AVQLLFGQAG
421LVVVDTAQIE NKEAYAPQIS LEGSRIVVQV PSTWCLKEDP ATMSLLQRSL DPEKTLGLVD
481VLYTAVLDLN RWRAGREQAL PCIQIQLQRE ICDFGNQADL PSGNGNKSSG GLQKTFSKLT
541SRFTKKASCT SSSSSTNYSI QNTPSKNIFI AGCSEEKAKM PGNIDTRLQS ILNIGNFPRT
601TDPSQSAQNS SNTVANGFLM ERRENFLHGD DGKDEKGMNL PTDQEMQEVI DFLSGFNMGQ
661SHQGSPLVTR HNSAATAMVT EQKAGAMQPQ QPSLPVPPPP RAPQAGAHTP LTPQPGLAPQ
721QQSPKQQQPQ VQYYQHLLQP IGPQQPPPQP RAPGKWVHGS SQQPAQAVGA GLSPLGQWPG
781ISDLSSDLYS LGLVSSYMDN VMSEVLGQKP QGPRNNTWPN RDQSDGVFGM LGEILPFDPA
841VGSDPEFARY VAGVSQAMQQ KRQAQHGRRP GNPRGNWPPM DDAHRTWPFP EFFTEGDGLH
901GGWSGAQGDS ASSSDETSSA NGDSLFSMFS GPDLVAAVKQ RRKHSSGEQD TSTLPSPPLL
961TTVEDVNQDN KTKTWPPKAP WQHPSPLPST LPSPSAPLYA VTSPGSQWND TMQMLQSPVW
1021AATNDCSAAA FSYVQTPPQP PPPPAHKAAP KGFKAFPGKG ERRPAYLPQY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1070 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.