LSBio
KIAA0284 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA0284 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CEP170B antibodies →
$725.00each
Catalog Number: LS-G124250-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0284(CEP170B) (NM_001112726), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centrosomal protein 170B | CEP170B | CEP170R | FAM68C | KIAA0284 | Protein KIAA0284
Origin Species
Human
NCBI GenBank
AB006622
SwissProt
Q9Y4F5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
167.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSATSWFLVS SSGARHRLPR ELIFVGREEC ELMLQSRSVD KQHAVINYDQ DRDEHWVKDL
61GSLNGTFVND MRIPDQKYVT LKLNDVIRFG YDSNMYVLER VQHRVPEEAL KHEKYTSQLQ
121VSVKGLAPKR SEALPEHTPY CEASNPRPEK GDRRPGTEAA SYRTPLYGQP SWWGEDDGST
181LPDAQRQGEP YPERPKGPVQ QDGELHGFRA PAEPQGCSFR REPSYFEIPT KETPQPSQPP
241EVPAHEMPTK DAEAGGGGAA PVVQSHASFT IEFDDCSPGK MKIKDHITKF SLRQRRPPGK
301EATPGEMVSA ETKVADWLVQ NDPSLLHRVG PGDDRHSTKS DLPVHTRTLK GHKHEDGTQS
361DSEDPLAKAA SAAGVPLEAS GEQVRLQRQI KRDPQELLHN QQAFVIEFFD EDTPRKKRSQ
421SFTHSPSGDP KADKRRGPTP ADRDRPSVPA PVQAGGRSSG PQRAGSLKRE KTEERLGSPS
481PASRTPARPF GSVGRRSRLA QDFMAQCLRE SSPAARPSPE KVPPVLPAPL TPHGTSPVGP
541PTPPPAPTDP QLTKARKQEE DDSLSDAGTY TIETEAQDTE VEEARKMIDQ VFGVLESPEL
601SRASSATFRP VIRGDRDESD DGGVAQRMAL LQEFASRPLG AAPQAEHQGL PVPGSPGGQK
661WVSRWASLAD SYSDPGLTED GLGRRGGEPE GSLPVRMRRR LPQLPSERAD SPAGPESSRR
721SGPGPPELDS EQPSRLFGQE ELDPDSLSDA SGSDGGRGPE PGVEPQDSRR RSPQEGPTWS
781RGRRSPRAPG EPTPASFFIG DQNGDAVLSR KPLAAPGDGE GLGQTAQPSP PARDGVYVSA
841NGRMVIQLRP GRSPEPDGPA PAFLRQESFT KEPASGPPAP GKPPHISSHP LLQDLAATRA
901ARMDFHSQDT HLILKETETA LAALEARLLS NSVDAECEGG STPRPPEDAL SGDSDVDTAS
961TVSLRSGKSG PSPTTPQPLR AQKEMSPSPP AAQDPGGTAL VSAREQSSER QHHPLGPTDM
1021GRGEPVRRSA IRRGHRPRGS LDWPSEERGP VLAHLPSSDV MASNHETPEA TGAGRLGSRR
1081KPAAPPPSPA AREEQSRSSA SSQKGPQALT RSNSLSTPRP TRASRLRRAR LGDASDTEAA
1141DGERGSLGNP EPVGRPAAEQ AKKLSRLDIL AMPRKRAGSF TGTSDPEAAP ARTSFSGRSV
1201ELCCASRKPT MAEARAVSRK AANTATTTGP RQPFSRARSG SARYTSTTQT PRAGSSSRAR
1261SRAPGPRDTD DDEEEPDPYG FIVQTAEIAE IARLSQTLVK DVAILAQEIH DVAGDGDTLG
1321SSEPAHSASL SNMPSTPAST ISAREELVQR IPEASLNFQK VPPGSLNSRD FDQNMNDSCE
1381DALANKTRPR NREEVIFDNL MLNPVSQLSQ AIRENTEHLA EKMKILFQNT GRAWEDLEAR
1441INAENEVPIL KTSNKEISSI LKELRRVQKQ LEVINAIVDP SGSLDLLTGN RSLASSAQPG
1501LGKGRVAAQS PPSPASAEAL LPALPLRNFP QRASCGPPSL PDPTFLPDAE RFLI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.