Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0226 / RUBICON Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113772-100
Catalog Number: LS-G113772
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Rubicon(KIAA0226) (NM_014687), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Baron | KIAA0226 | RUBICON | Rundataxin
- Origin Species
- Human
- NCBI GenBank
- D86979
- SwissProt
- Q92622
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRPEGAGMEL GGGEERLPEE SRREHWQLLG NLKTTVEGLV STNSPNVWSK YGGLERLCRD61MQSILYHGLI RDQACRRQTD YWQFVKDIRW LSPHSALHVE KFISVHENDQ SSADGASERA121VAELWLQHSL QYHCLSAQLR PLLGDRQYIR KFYTDAAFLL SDAHVTAMLQ CLEAVEQNNP181RLLAQIDASM FARKHESPLL VTKSQSLTAL PSSTYTPPNS YAQHSYFGSF SSLHQSVPNN241GSERRSTSFP LSGPPRKPQE SRGHVSPAED QTIQAPPVSV SALARDSPLT PNEMSSSTLT301SPIEASWVSS QNDSPGDASE GPEYLAIGNL DPRGRTASCQ SHSSNAESSS SNLFSSSSSQ361KPDSAASSLG DQEGGGESQL SSVLRRSSFS EGQTLTVTSG AKKSHIRSHS DTSIASRGAP421ESCNDKAKLR GPLPYSGQSS EVSTPSSLYM EYEGGRYLCS GEGMFRRPSE GQSLISYLSE481QDFGSCADLE KENAHFSISE SLIAAIELMK CNMMSQCLEE EEVEEEDSDR EIQELKQKIR541LRRQQIRTKN LLPMYQEAEH GSFRVTSSSS QFSSRDSAQL SDSGSADEVD EFEIQDADIR601RNTASSSKSF VSSQSFSHCF LHSTSAEAVA MGLLKQFEGM QLPAASELEW LVPEHDAPQK661LLPIPDSLPI SPDDGQHADI YKLRIRVRGN LEWAPPRPQI IFNVHPAPTR KIAVAKQNYR721CAGCGIRTDP DYIKRLRYCE YLGKYFCQCC HENAQMAIPS RVLRKWDFSK YYVSNFSKDL781LIKIWNDPLF NVQDINSALY RKVKLLNQVR LLRVQLCHMK NMFKTCRLAK ELLDSFDTVP841GHLTEDLHLY SLNDLTATRK GELGPRLAEL TRAGATHVER CMLCQAKGFI CEFCQNEDDI901IFPFELHKCR TCEECKACYH KACFKSGSCP RCERLQARRE ALARQSLESY LSDYEEEPAE961ALALEAAVLE ATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
972 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.