Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0153 / TTLL12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101301-100
Catalog Number: LS-G101301
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL12 (NM_015140), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ526I14.2 | KIAA0153 | TTLL12
- Origin Species
- Human
- NCBI GenBank
- NM_015140
- SwissProt
- Q14166
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAERGPERR PAERSSPGQT PEEGAQALAE FAALHGPALR ASGVPERYWG RLLHKLEHEV61FDAGEVFGIM QVEEVEEEED EAAREVRKQQ PNPGNELCYK VIVTRESGLQ AAHPNSIFLI121DHAWTCRVEH ARQQLQQVPG LLHRMANLMG IEFHGELPST EAVALVLEEM WKFNQTYQLA181HGTAEEKMPV WYIMDEFGSR IQHADVPSFA TAPFFYMPQQ VAYTLLWPLR DLDTGEEVTR241DFAYGETDPL IRKCMLLPWA PTDMLDLSSC TPEPPAEHYQ AILEENKEKL PLDINPVVHP301HGHIFKVYTD VQQVASSLTH PRFTLTQSEA DADILFNFSH FKDYRKLSQE RPGVLLNQFP361CENLLTVKDC LASIARRAGG PEGPPWLPRT FNLRTELPQF VSYFQQRERW GEDNHWICKP421WNLARSLDTH VTKSLHSIIR HRESTPKVVS KYIESPVLFL REDVGKVKFD IRYIVLLRSV481RPLRLFVYDV FWLRFSNRAF ALNDLDDYEK HFTVMNYDPD VVLKQVHCEE FIPEFEKQYP541EFPWTDVQAE IFRAFTELFQ VACAKPPPLG LCDYPSSRAM YAVDLMLKWD NGPDGRRVMQ601PQILEVNFNP DCERACRYHP TFFNDVFSTL FLDQPGGCHV TCLVTRTRPL EQKLISEEDL661AANDILDYKD DDDKV
675 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.