Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0020 / PEN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113631-100
Catalog Number: LS-G113631
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0020(PUM3) (NM_014878), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HA-8 | HPUF-A | HLA-HA8 | KIAA0020 | PEN | RP11-526D20.2 | XTP5 | Penguin homolog | PUF6
- Origin Species
- Human
- NCBI GenBank
- NM_014878
- SwissProt
- Q15397
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVKGKKQFT GKNTKTAQEK NRFHKNSDSG SSKTFPTRKV AKEGGPKVTS RNFEKSITKL61GKKGVKQFKN KQQGDKSPKN KFQPANKFNK KRKFQPDGRS DESAAKKPKW DDFKKKKKEL121KQSRQLSDKT NYDIVVRAKQ MWEILRRKDC DKEKRVKLMS DLQKLIQGKI KTIAFAHDST181RVIQCYIQYG NEEQRKQAFE ELRDDLVELS KAKYSRNIVK KFLMYGSKPQ IAEIIRSFKG241HVRKMLRHAE ASAIVEYAYN DKAILEQRNM LTEELYGNTF QLYKSADHPT LDKVLELQPE301KLELIMDEMK QILTPMAQKE AVIKHSLVHK VFLDFFTYAP PKLRSEMIEA IREAVVYLAH361THDGARVAMH CLWHGTPKDR KVIVKTMKTY VEKVANGQYS HLVLLAAFDC IDDTKLVKQI421IISEIISSLP SIVNDKYGRK VLLYLLSPRD PAHTVREIIE VLQKGDGNAH SKKDTEVRRR481ELLESISPAL LSYLQEHAQE VVLDKSACVL VSDILGSATG DVQPTMNAIA SLAATGLHPG541GKDGELHIAE HPAGHLVLKW LIEQDKKMKE NGREGCFAKT LVEHVGMKNL KSWASVNRGA601IILSSLLQSC DLEVANKVKA ALKSLIPTLE KTKSTSKGIE ILLEKLSTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
648 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.