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KHNYN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KHNYN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KHNYN antibodies →
$504.00each
Catalog Number: LS-G113306-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0323(KHNYN) (NM_015299), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KH and NYN domain containing | KIAA0323 | KHNYN | Protein KHNYN
Origin Species
Human
NCBI GenBank
NM_015299
SwissProt
O15037

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTWGARPAS PDRFAVSAEA ENKVREQQPH VERIFSVGVS VLPKDCPDNP HIWLQLEGPK
61ENASRAKEYL KGLCSPELQD EIHYPPKLHC IFLGAQGFFL DCLAWSTSAH LVPRAPGSLM
121ISGLTEAFVM AQSRVEELAE RLSWDFTPGP SSGASQCTGV LRDFSALLQS PGDAHREALL
181QLPLAVQEEL LSLVQEASSG QGPGALASWE GRSSALLGAQ CQGVRAPPSD GRESLDTGSM
241GPGDCRGARG DTYAVEKEGG KQGGPREMDL GWKELPGEEA WEREVALRPQ SVGGGARESA
301PLKGKALGKE EIALGGGGFC VHREPPGAHG SCHRAAQSRG ASLLQRLHNG NASPPRVPSP
361PPAPEPPWHC GDRGDCGDRG DVGDRGDKQQ GMARGRGPQW KRGARGGNLV TGTQRFKEAL
421QDPFTLCLAN VPGQPDLRHI VIDGSNVAMV HGLQHYFSSR GIAIAVQYFW DRGHRDITVF
481VPQWRFSKDA KVRESHFLQK LYSLSLLSLT PSRVMDGKRI SSYDDRFMVK LAEETDGIIV
541SNDQFRDLAE ESEKWMAIIR ERLLPFTFVG NLFMVPDDPL GRNGPTLDEF LKKPARTQGS
601SKAQHPSRGF AEHGKQQQGR EEEKGSGGIR KTRETERLRR QLLEVFWGQD HKVDFILQRE
661PYCRDINQLS EALLSLNF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

678 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.