Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KDM8 / JMJD5 / FLJ13798 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126662-100
Catalog Number: LS-G126662
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JMJD5(KDM8) (NM_001145348), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLJ13798 | JMJD5 | Jumonji domain containing 5 | KDM8 | Lysine-specific demethylase 8
- Origin Species
- Human
- NCBI GenBank
- NM_024773
- SwissProt
- Q8N371
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSREKCSPGE GAEEGRGSEA SGLKASAGHG TEPAGGGPMA GDTHCPAEPL AREGTLWEAL61RALLPHSKED LKLDLGEKVE RSVVTLLQRA TELFYEGRRD ECLQSSEVIL DYSWEKLNTG121TWQDVDKDWR RVYAIGCLLK ALCLCQAPED ANTVAAALRV CDMGLLMGAA ILGDILLKVA181AILQTHLPGK RPARGSLPEQ PCTKKARADH GLIPDVKLEK TVPRLHRPSL QHFREQFLVP241GRPVILKGVA DHWPCMQKWS LEYIQEIAGC RTVPVEVGSR YTDEEWSQTL MTVNEFISKY301IVNEPRDVGY LAQHQLFDQI PELKQDISIP DYCSLGDGEE EEITINAWFG PQGTISPLHQ361DPQQNFLVQV MGRKYIRLYS PQESGALYPH DTHLLHNTSQ VDVENPDLEK FPKFAKAPFL421SCILSPGEIL FIPVKYWHYV RALDLSFSVS FWWSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
454 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.