Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KDM5D / JARID1D Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128849-100
Catalog Number: LS-G128849
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KDM5D (NM_001146705), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- H-Y | HYA | HY | JARID1D | Histocompatibility Y antigen | Histone demethylase JARID1D | KIAA0234 | Protein SmcY | SMCY | Smcy homolog | Y-linked (mouse) | Y-linked | KDM5D | Lysine-specific demethylase 5D | SMC homolog | Y chromosome
- Origin Species
- Human
- NCBI GenBank
- NM_004653
- SwissProt
- Q9BY66
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 177.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPGCDEFLP PPECPVFEPS WAEFQDPLGY IAKIRPIAEK SGICKIRPPA DWQPPFAVEV61DNFRFTPRVQ RLNELEAQTR VKLNYLDQIA KFWEIQGSSL KIPNVERKIL DLYSLSKIVI121EEGGYEAICK DRRWARVAQR LHYPPGKNIG SLLRSHYERI IYPYEMFQSG ANHVQCNTHP181FDNEVKDKEY KPHSIPLRQS VQPSKFSSYS RRAKRLQPDP EPTEEDIEKH PELKKLQIYG241PGPKMMGLGL MAKDKDKTVH KKVTCPPTVT VKDEQSGGGN VSSTLLKQHL SLEPCTKTTM301QLRKNHSSAQ FIDSYICQVC SRGDEDDKLL FCDGCDDNYH IFCLLPPLPE IPRGIWRCPK361CILAECKQPP EAFGFEQATQ EYSLQSFGEM ADSFKSDYFN MPVHMVPTEL VEKEFWRLVS421SIEEDVTVEY GADIHSKEFG SGFPVSNSKQ NLSPEEKRQS LTVLTRLISS FWAQAVLPPW481PPKVLGLQEY ATSGWNLNVM PVLDQSVLCH INADISGMKV PWLYVGMVFS AFCWHIEDHW541SYSINYLHWG EPKTWYGVPS LAAEHLEEVM KMLTPELFDS QPDLLHQLVT LMNPNTLMSH601GVPVVRTNQC AGEFVITFPR AYHSGFNQGY NFAEAVNFCT ADWLPAGRQC IEHYRRLRRY661CVFSHEELIC KMAAFPETLD LNLAVAVHKE MFIMVQEERR LRKALLEKGV TEAEREAFEL721LPDDERQCIK CKTTCFLSAL ACYDCPDGLV CLSHINDLCK CSSSRQYLRY RYTLDELPTM781LHKLKIRAES FDTWANKVRV ALEVEDGRKR SFEELRALES EARERRFPNS ELLQRLKNCL841SEVEACIAQV LGLVSGQVAR MDTPQLTLTE LRVLLEQMGS LPCAMHQIGD VKDVLEQVEA901YQAEAREALA TLPSSPGLLR SLLERGQQLG VEVPEAHQLQ QQVEQAQWLD EVKQALAPSA961HRGSLVIMQG LLVMGAKIAS SPSVDKARAE LQELLTIAER WEEKAHFCLE ARQKHPPATL1021EAIIRETENI PVHLPNIQAL KEALTKAQAW IADVDEIQNG DHYPCLDDLE GLVAVGRDLP1081VGLEELRQLE LQVLTAHSWR EKASKTFLKK NSCYTLLEVL CPCADAGSDS TKRSRWMEKA1141LGLYQCDTEL LGLSAQDLRD PGSVIVAFKE GEQKEKEGIL QLRRTNSAKP SPLAPSLMAS1201SPTSICVCGQ VPAGVGVLQC DLCQDWFHGQ CVSVPHLLTS PKPSLTSSPL LAWWEWDTKF1261LCPLCMRSRR PRLETILALL VALQRLPVRL PEGEALQCLT ERAIGWQDRA RKALASEDVT1321ALLRQLAELR QQLQAKPRPE EASVYTSATA CDPIREGSGN NISKVQGLLE NGDSVTSPEN1381MAPGKGSDLE LLSSLLPQLT GPVLELPEAI RAPLEELMME GDLLEVTLDE NHSIWQLLQA1441GQPPDLDRIR TLLELEKFEH QGSRTRSRAL ERRRRRQKVD QGRNVENLVQ QELQSKRARS1501SGIMSQVGRE EEHYQEKADR ENMFLTPSTD HSPFLKGNQN SLQHKDSGSS AACPSLMPLL1561QLSYSDEQQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1570 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.