Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KDM2B / FBXL10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122198-100
Catalog Number: LS-G122198
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KDM2B (NM_001005366), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CXXC2 | Fbl10 | F-box/LRR-repeat protein 10 | JHDM1B | F-box protein FBL10 | KDM2B | FBXL10 | Lysine-specific demethylase 2B | PCCX2 | Protein JEMMA
- Origin Species
- Human
- NCBI GenBank
- AL133572
- SwissProt
- Q8NHM5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 144.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAEKDSGRR LRPIDRQRYD ENEDLSDVEE IVSVRGFSLE EKLRSQLYQG DFVHAMEGKD61FNYEYVQREA LRVPLIFREK DGLGIKMPDP DFTVRDVKLL VGSRRLVDVM DVNTQKGTEM121SMSQFVRYYE TPEAQRDKLY NVISLEFSHT KLEHLVKRPT VVDLVDWVDN MWPQHLKEKQ181TEATNAIAEM KYPKVKKYCL MSVKGCFTDF HIDFGGTSVW YHVFRGGKIF WLIPPTLHNL241ALYEEWVLSG KQSDIFLGDR VERCQRIELK QGYTFFIPSG WIHAVYTPVD SLVFGGNILH301SFNVPMQLRI YEIEDRTRVQ PKFRYPFYYE MCWYVLERYV YCVTQRSHLT QEYQRESMLI361DAPRKPSIDG FSSDSWLEME EEACDQQPQE EEEKDEEGEG RDRAPKPPTD GSTSPTSTPS421EDQEALGKKP KAPALRFLKR TLSNESEESV KSTTLAVDYP KTPTGSPATE VSAKWTHLTE481FELKGLKALV EKLESLPENK KCVPEGIEDP QALLEGVKNV LKEHADDDPS LAITGVPVVT541WPKKTPKNRA VGRPKGKLGP ASAVKLAANR TTAGARRRRT RCRKCEACLR TECGECHFCK601DMKKFGGPGR MKQSCIMRQC IAPVLPHTAV CLVCGEAGKE DTVEEEEGKF NLMLMECSIC661NEIIHPGCLK IKESEGVVND ELPNCWECPK CNHAGKTGKQ KRGPGFKYAS NLPGSLLKEQ721KMNRDNKEGQ EPAKRRSECE EAPRRRSDEH SKKVPPDGLL RRKSDDVHLR KKRKYEKPQE781LSGRKRLKPG KEDKLFRKKR RSWKNAEDRM ALANKPLRRF KQEPEDELPE APPKTRESDH841SRSSSPTAGP STEGAEGPEE KKKVKMRRKR RLPNKELSRE LSKELNHEIQ RTENSLANEN901QQPIKSEPES EGEEPKRPPG ICERPHRFSK GLNGTPRELR HQLGPSLRSP PRVISRPPPS961VSPPKCIQME RHVIRPPPIS PPPDSLPLDD GAAHVMHREV WMAVFSYLSH QDLCVCMRVC1021RTWNRWCCDK RLWTRIDLNH CKSITPLMLS GIIRRQPVSL DLSWTNISKK QLSWLINRLP1081GLRDLVLSGC SWIAVSALCS SSCPLLRTLD VQWVEGLKDA QMRDLLSPPT DNRPGQMDNR1141SKLRNIVELR LAGLDITDAS LRLIIRHMPL LSKLHLSYCN HVTDQSINLL TAVGTTTRDS1201LTEINLSDCN KVTDQCLSFF KRCGNICHID LRYCKQVTKE GCEQFIAEMS SFQGRSCSTT1261RLGDETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1265 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.