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KDM1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KDM1B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102394-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KDM1B(KDM1B) (NM_153042), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AOF1 | BA204B7.3 | C6orf193 | DJ298J15.2 | KDM1B | LSD2
Origin Species
Human
SwissProt
Q8NB78

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATPRGRTKK KASFDHSPDS LPLRSSGRQA KKKATETTDE DEDGGSEKKY RKCEKAGCTA
61TCPVCFASAS ERCAKNGYTS RWYHLSCGEH FCNECFDHYY RSHKDGYDKY TTWKKIWTSN
121GKTEPSPKAF MADQQLPYWV QCTKPECRKW RQLTKEIQLT PQIAKTYRCG MKPNTAIKPE
181TSDHCSLPED LEALTPQKCI PHIIVRGLVR IRCVQEVERI LYFMTRKGLI NTGVLSVGAD
241QYLLPKDYHN KSVIIIGAGP AGLAAARQLH NFGIKVTVLE AKDRIGGRVW DDKSFKGVTV
301GRGAQIVNGC INNPVALMCE QVSARSWDHN EFFAQFAGDH TLLTPGYSVI IEKLAEGLDI
361QLKSPVQCID YSGDEVQVTT TDGTGYSAQK VLVTVPLALL QKGAIQFNPP LSEKKMKAIN
421SLGAGIIEKI ALQFPYRFWD SKVQGADFFG HVPPSASKRG LFAVFYDMDP QKKHSVLMSV
481IAGEAVASVR TLDDKQVLQQ CMATLRELFK EQEVPDPTKY FVTRWSTDPW IQMAYSFVKT
541GGSGEAYDII AEDIQGTVFF AGEATNRHFP QTVTGAYLSG VREASKIAAF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.