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KDELC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KDELC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105702-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KDELC2 (NM_153705), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KDELC2
Origin Species
Human
NCBI GenBank
NM_153705
SwissProt
Q7Z4H8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRLPRALLL QLRLALLVAA GAPEVLVSAP RSLVWGPGLQ AAVVLPVRYF YLQAVNSEGQ
61NLTRSPAGET PFKVVVKSLS PKELVRIHVP KPLDRNDGTF LMRYRMYETV DEGLKIEVLY
121GDEHVAQSPY ILKGPVYHEY CECPEDPQAW QKTLSCPTKE PQIAKDFASF PSINLQQMLK
181EVPKRFGDER GAIVHYTILN NHVYRRSLGK YTDFKMFSDE ILLSLTRKVL LPDLEFYVNL
241GDWPLEHRKV NGTPSPIPII SWCGSLDSRD VVLPTYDITH SMLEAMRGVT NDLLSIQGNT
301GPSWINKTER AFFRGRDSLE ERLQLVQLSK ENPQLLDAGI TGYFFFQEKE KELGKAKLMG
361FFDFFKYKYQ VNVDGTVAAY RYPYLMLGDS LVLKQDSPYY EHFYMALEPW KHYVPIKRNL
421SDLLEKVKWA KENDEEAKKI AKEGQLMARD LLQPHRLYCY YYQVLQKYAE RQSSKPEVRD
481GMELVPQPED STAICQCHRK KPSREEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.