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KCTD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KCTD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112851-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCTD3 (NM_016121), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KCTD3 | NY-REN-45 | NY-REN-45 antigen
Origin Species
Human
NCBI GenBank
NM_016121
SwissProt
Q9Y597

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGGHCGSVP AAAAGSGEIV QLNVGGTRFS TSRQTLMWIP DSFFSSLLSG RISTLRDETG
61AIFIDRDPAA FAPILNFLRT KELDLRGVSI NVLRHEAEFY GITPLVRRLL LCEELERSSC
121GSVLFHGYLP PPGIPSRKIN NTVRSADSRN GLNSTEGEAR GNGTQPVLSG TGEETVRLGF
181PVDPRKVLIV AGHHNWIVAA YAHFAVCYRI KESSGWQQVF TSPYLDWTIE RVALNAKVVG
241GPHGDKDKMV AVASESSIIL WSVQDGGSGS EIGVFSLGVP VDALFFIGNQ LVATSHTGKV
301GVWNAVTQHW QVQDVVPITS YDTAGSFLLL GCNNGSIYYI DMQKFPLRMK DNDLLVTELY
361HDPSNDAITA LSVYLTPKTS VSGNWIEIAY GTSSGAVRVI VQHPETVGSG PQLFQTFTVH
421RSPVTKIMLS EKHLVSVCAD NNHVRTWTVT RFRGMISTQP GSTPLASFKI LSLEETESHG
481SYSSGNDIGP FGERDDQQVF IQKVVPITNK LFVRLSSTGK RICEIQAVDC TTISSFTVRE
541CEGSSRMGSR PRRYLFTGHT NGSIQMWDLT TAMDMVNKSE DKDVGGPTEE ELLKLLDQCD
601LSTSRCATPN ISPATSVVQH SHLRESNSSL QLQHHDTTHE AATYGSMRPY RESPLLARAR
661RTESFHSYRD FQTINLNRNV ERAVPENGNL GPIQAEVKGA TGECNISERK SPGVEIKSLR
721ELDSGLEVHK IAEGFSESKK RSSEDENENK IEFRKKGGFE GGGFLGRKKV PYLASSPSTS
781DGGTDSPGTA SPSPTKTTPS PRHKKSDSSG QEYSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

815 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.