Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCP / CRIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G125570-100
Catalog Number: LS-G125570
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCP (NM_001135914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRIM-2 | Cysteine-rich BMP regulator 2 | CRIM2 | KCP | KCP-1 | Kielin/chordin-like protein 1 | KCP1 | Kielin/chordin-like protein | Kielin | NET67
- Origin Species
- Human
- SwissProt
- Q6ZWJ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 159.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGVGAAALS LLLHLGALAL AAGAEGGAVP REPPGQQTTA HSSVLAGNSQ EQWHPLREWL61GRLEAAVMEL REQNKDLQTR VRQLESCECH PASPQCWGLG RAWPEGARWE PDACTACVCQ121DGAAHCGPQA HLPHCRGCSQ NGQTYGNGET FSPDACTTCR CLTGAVQCQG PSCSELNCLE181SCTPPGECCP ICCTEGGSHW EHGQEWTTPG DPCRICRCLE GHIQCRQREC ASLCPYPARP241LPGTCCPVCD GCFLNGREHR SGEPVGSGDP CSHCRCANGS VQCEPLPCPP VPCRHPGKIP301GQCCPVCDGC EYQGHQYQSQ ETFRLQERGL CVRCSCQAGE VSCEEQECPV TPCALPASGR361QLCPACELDG EEFAEGVQWE PDGRPCTACV CQDGVPKCGA VLCPPAPCQH PTQPPGACCP421SCDSCTYHSQ VYANGQNFTD ADSPCHACHC QDGTVTCSLV DCPPTTCARP QSGPGQCCPR481CPDCILEEEV FVDGESFSHP RDPCQECRCQ EGHAHCQPRP CPRAPCAHPL PGTCCPNDCS541GCAFGGKEYP SGADFPHPSD PCRLCRCLSG NVQCLARRCV PLPCPEPVLL PGECCPQCPA601PAGCPRPGAA HARHQEYFSP PGDPCRRCLC LDGSVSCQRL PCPPAPCAHP RQGPCCPSCD661GCLYQGKEFA SGERFPSPTA ACHLCLCWEG SVSCEPKACA PALCPFPARG DCCPDCDGCE721YLGESYLSNQ EFPDPREPCN LCTCLGGFVT CGRRPCEPPG CSHPLIPSGH CCPTCQGCRY781HGVTTASGET LPDPLDPTCS LCTCQEGSMR CQKKPCPPAL CPHPSPGPCF CPVCHSCLSQ841GREHQDGEEF EGPAGSCEWC RCQAGQVSCV RLQCPPLPCK LQVTERGSCC PRCRGCLAHG901EEHPEGSRWV PPDSACSSCV CHEGVVTCAR IQCISSCAQP RQGPHDCCPQ CSDCEHEGRK961YEPGESFQPG ADPCEVCICE PQPEGPPSLR CHRRQCPSLV GCPPSQLLPP GPQHCCPTCA1021EALSNCSEGL LGSELAPPDP CYTCQCQDLT WLCIHQACPE LSCPLSERHT PPGSCCPVCR1081APTQSCVHQG REVASGERWT VDTCTSCSCM AGTVRCQSQR CSPLSCGPDK APALSPGSCC1141PRCLPRPASC MAFGDPHYRT FDGRLLHFQG SCSYVLAKDC HSGDFSVHVT NDDRGRSGVA1201WTQEVAVLLG DMAVRLLQDG AVTVDGHPVA LPFLQEPLLY VELRGHTVIL HAQPGLQVLW1261DGQSQVEVSV PGSYQGRTCG LCGNFNGFAQ DDLQGPEGLL LPSEAAFGNS WQVSEGLWPG1321RPCSAGREVD PCRAAGYRAR REANARCGVL KSSPFSRCHA VVPPEPFFAA CVYDLCACGP1381GSSADACLCD ALEAYASHCR QAGVTPTWRG PTLCVVGCPL ERGFVFDECG PPCPRTCFNQ1441HIPLGELAAH CVRPCVPGCQ CPAGLVEHEA HCIPPEACPQ VLLTGDQPLG ARPSPSREPQ1501ETPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1503 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.