Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNV1 / Kv8.1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113988-100
Catalog Number: LS-G113988
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNV1 (NM_014379), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KCNV1 | Potassium channel Kv8.1 | KV2.3 | HNKA | KCNB3 | KV8.1
- Origin Species
- Human
- NCBI GenBank
- NM_014379
- SwissProt
- Q6PIU1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSSGRALLD SPLDSGSLTS LGSSVFCSEG EGEPLALGDC FTVNVGGSRF VLSQQALSCF61PHTRLGKLAV VVASYRRPGA LAAVPSPLEL CDDANPVDNE YFFDRSSQAF RYVLHYYRTG121RLHVMEQLCA LSFLQEIQYW GIDELSIDSC CRDRYFRRKE LSETLDFKKD TEDQESQHES181EQDFSQGPCP TVRQKLWNIL EKPGSSTAAR IFGVISIIFV VVSIINMALM SAELSWLDLQ241LLEILEYVCI SWFTGEFVLR FLCVRDRCRF LRKVPNIIDL LAILPFYITL LVESLSGSQT301TQELENVGRI VQVLRLLRAL RMLKLGRHST GLRSLGMTIT QCYEEVGLLL LFLSVGISIF361STVEYFAEQS IPDTTFTSVP CAWWWATTSM TTVGYGDIRP DTTTGKIVAF MCILSGILVL421ALPIAIINDR FSACYFTLKL KEAAVRQREA LKKLTKNIAT DSYISVNLRD VYARSIMEML481RLKGRERAST RSSGGDDFWFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
500 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.