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KCNQ4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KCNQ4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105504-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNQ4 (NM_172163), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DFNA2A | DFNA2 | KV7.4 | KQT-like 4 | KCNQ4 | Potassium channel KQT-like 4
Origin Species
Human
NCBI GenBank
NM_004700
SwissProt
P56696

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAPPRRLG LGPPPGDAPR AELVALTAVQ SEQGEAGGGG SPRRLGLLGS PLPPGAPLPG
61PGSGSGSACG QRSSAAHKRY RRLQNWVYNV LERPRGWAFV YHVFIFLLVF SCLVLSVLST
121IQEHQELANE CLLILEFVMI VVFGLEYIVR VWSAGCCCRY RGWQGRFRFA RKPFCVIDFI
181VFVASVAVIA AGTQGNIFAT SALRSMRFLQ ILRMVRMDRR GGTWKLLGSV VYAHSKELIT
241AWYIGFLVLI FASFLVYLAE KDANSDFSSY ADSLWWGTIT LTTIGYGDKT PHTWLGRVLA
301AGFALLGISF FALPAGILGS GFALKVQEQH RQKHFEKRRM PAANLIQAAW RLYSTDMSRA
361YLTATWYYYD SILPSFSSRM GIKDRIRMGS SQRRTGPSKQ HLAPPTMPTS PSSEQVGEAT
421SPTKVQKSWS FNDRTRFRAS LRLKPRTSAE DAPSEEVAEE KSYQCELTVD DIMPAVKTVI
481RSIRILKFLV AKRKFKETLR PYDVKDVIEQ YSAGHLDMLG RIKSLQTRVD QIVGRGPGDR
541KAREKGDKGP SDAEVVDEIS MMGRVVKVEK QVQSIEHKLD LLLGFYSRCL RSGTSASLGA
601VQVPLFDPDI TSDYHSPVDH EDISVSAQTL SISRSVSTNM DTRR
LEQKLISEEDLAANDILDYKDDDDKV

644 aa + 26 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.