Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNN3 / SK3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99701-100
Catalog Number: LS-G99701
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNN3 (NM_002249), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KCa2.3 | KCNN3 | HSK3 | SKCa 3 | SKCA3 | K3 | SK3
- NCBI GenBank
- NM_002249
- SwissProt
- Q9UGI6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTSGHFHDS GVGDLDEDPK CPCPSSGDEQ QQQQQQQQQQ QPPPPAPPAA PQQPLGPSLQ61PQPPQLQQQQ QQQQQQQQQQ QQQQPPHPLS QLAQLQSQPV HPGLLHSSPT AFRAPPSSNS121TAILHPSSRQ GSQLNLNDHL LGHSPSSTAT SGPGGGSRHR QASPLVHRRD SNPFTEIAMS181SCKYSGGVMK PLSRLSASRR NLIEAETEGQ PLQLFSPSNP PEIVISSRED NHAHQTLLHH241PNATHNHQHA GTTASSTTFP KANKRKNQNI GYKLGHRRAL FEKRKRLSDY ALIFGMFGIV301VMVIETELSW GLYSKDSMFS LALKCLISLS TIILLGLIIA YHTREVQLFV IDNGADDWRI361AMTYERILYI SLEMLVCAIH PIPGEYKFFW TARLAFSYTP SRAEADVDII LSIPMFLRLY421LIARVMLLHS KLFTDASSRS IGALNKINFN TRFVMKTLMT ICPGTVLLVF SISLWIIAAW481TVRVCERYHD QQDVTSNFLG AMWLISITFL SIGYGDMVPH TYCGKGVCLL TGIMGAGCTA541LVVAVVARKL ELTKAEKHVH NFMMDTQLTK RIKNAAANVL RETWLIYKHT KLLKKIDHAK601VRKHQRKFLQ AIHQLRSVKM EQRKLSDQAN TLVDLSKMQN VMYDLITELN DRSEDLEKQI661GSLESKLEHL TASFNSLPLL IADTLRQQQQ QLLSAIIEAR GVSVAVGTTH TPISDSPIGV721SSTSFPTPYT SSSSCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
735 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.