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KCNN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KCNN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G99700-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNN1 (NM_002248), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KCNN1 | SK1 | SKCa 1 | Hsk1 | KCa2.1 | SK | SKCA1
NCBI GenBank
NM_002248
SwissProt
Q92952

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSHSYNGSV GRPLGSGPGA LGRDPPDPEA GHPPQPPHSP GLQVVVAKSE PARPSPGSPR
61GQPQDQDDDE DDEEDEAGRQ RASGKPSNVG HRLGHRRALF EKRKRLSDYA LIFGMFGIVV
121MVTETELSWG VYTKESLYSF ALKCLISLST AILLGLVVLY HAREIQLFMV DNGADDWRIA
181MTCERVFLIS LELAVCAIHP VPGHYRFTWT ARLAFTYAPS VAEADVDVLL SIPMFLRLYL
241LGRVMLLHSK IFTDASSRSI GALNKITFNT RFVMKTLMTI CPGTVLLVFS ISSWIIAAWT
301VRVCERYHDK QEVTSNFLGA MWLISITFLS IGYGDMVPHT YCGKGVCLLT GIMGAGCTAL
361VVAVVARKLE LTKAEKHVHN FMMDTQLTKR VKNAAANVLR ETWLIYKHTR LVKKPDQARV
421RKHQRKFLQA IHQAQKLRSV KIEQGKLNDQ ANTLTDLAKT QTVMYDLVSE LHAQHEELEA
481RLATLESRLD ALGASLQALP GLIAQAIRPP PPPLPPRPGP GPQDQAARSS PCRWTPVAPS
541DCG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.