Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNMA1 / BK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99699-100
Catalog Number: LS-G99699
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNMA1 (NM_002247), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA205K10.1 | BKCA alpha subunit | BK channel | BKCA alpha | BKTM | HSlo | KCNMA1 | K(VCA)alpha | KCa1.1 | Maxi-K channel HSLO | MaxiK | KCNMA | SAKCA | SLO-ALPHA | Slowpoke homolog | Stretch-activated Kca channel | Slo homolog | BK channel alpha subunit | Maxi K channel | MSlo | MSLO1 | SLO | SLO1
- NCBI GenBank
- NM_002247
- SwissProt
- Q12791
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MANGGGGGGG SSGGGGGGGG SSLRMSSNIH ANHLSLDASS SSSSSSSSSS SSSSSSSSSS61VHEPKMDALI IPVTMEVPCD SRGQRMWWAF LASSMVTFFG GLFIILLWRT LKYLWTVCCH121CGGKTKEAQK INNGSSQADG TLKPVDEKEE AVAAEVGWMT SVKDWAGVMI SAQTLTGRVL181VVLVFALSIG ALVIYFIDSS NPIESCQNFY KDFTLQIDMA FNVFFLLYFG LRFIAANDKL241WFWLEVNSVV DFFTVPPVFV SVYLNRSWLG LRFLRALRLI QFSEILQFLN ILKTSNSIKL301VNLLSIFIST WLTAAGFIHL VENSGDPWEN FQNNQALTYW ECVYLLMVTM STVGYGDVYA361KTTLGRLFMV FFILGGLAMF ASYVPEIIEL IGNRKKYGGS YSAVSGRKHI VVCGHITLES421VSNFLKDFLH KDRDDVNVEI VFLHNISPNL ELEALFKRHF TQVEFYQGSV LNPHDLARVK481IESADACLIL ANKYCADPDA EDASNIMRVI SIKNYHPKIR IITQMLQYHN KAHLLNIPSW541NWKEGDDAIC LAELKLGFIA QSCLAQGLST MLANLFSMRS FIKIEEDTWQ KYYLEGVSNE601MYTEYLSSAF VGLSFPTVCE LCFVKLKLLM IAIEYKSANR ESRILINPGN HLKIQEGTLG661FFIASDAKEV KRAFFYCKAC HDDITDPKRI KKCGCKRLED EQPSTLSPKK KQRNGGMRNS721PNTSPKLMRH DPLLIPGNDQ IDNMDSNVKK YDSTGMFHWC APKEIEKVIL TRSEAAMTVL781SGHVVVCIFG DVSSALIGLR NLVMPLRASN FHYHELKHIV FVGSIEYLKR EWETLHNFPK841VSILPGTPLS RADLRAVNIN LCDMCVILSA NQNNIDDTSL QDKECILASL NIKSMQFDDS901IGVLQANSQG FTPPGMDRSS PDNSPVHGML RQPSITTGVN IPIITELVND TNVQFLDQDD961DDDPDTELYL TQPFACGTAF AVSVLDSLMS ATYFNDNILT LIRTLVTGGA TPELEALIAE1021ENALRGGYST PQTLANRDRC RVAQLALLDG PFADLGDGGC YGDLFCKALK TYNMLCFGIY1081RLRDAHLSTP SQCTKRYVIT NPPYEFELVP TDLIFCLMQF DHNAGQSRAS LSHSSHSSQS1141SSKKSSSVHS IPSTANRQNR PKSRESRDKQ KYVQEERLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1178 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.