Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNK4 / TRAAK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108295-100
Catalog Number: LS-G108295
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNK4 (NM_033310), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- K2P4.1 potassium channel | K2p4.1 | KCNK4 | KT4.1 | Two pore K(+) channel KT4.1 | TRAAK1 | Two pore K+ channel KT4.1 | TRAAK
- Origin Species
- Human
- NCBI GenBank
- AF247042
- SwissProt
- Q9NYG8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAGDAGASA ESAVTTAPQE PPARPLQAGS GAGPAPGRAM RSTTLLALLA LVLLYLVSGA61LVFRALEQPH EQQAQRELGE VREKFLRAHP CVSDQELGLL IKEVADALGG GADPETNSTS121NSSHSAWDLG SAFFFSGTII TTIGYGNVAL RTDAGRLFCI FYALVGIPLF GILLAGVGDR181LGSSLRHGIG HIEAIFLKWH VPPELVRVLS AMLFLLIGCL LFVLTPTFVF CYMEDWSKLE241AIYFVIVTLT TVGFGDYVAG ADPRQDSPAY QPLVWFWILL GLAYFASVLT TIGNWLRVVS301RRTRAEMGGL TAQAASWTGT VTARVTQRAG PAAPPPEKEQ PLLPPPPCPA QPLGRPRSPS361PPEKAQPPSP PTASALDYPS ENLAFIDESS DTQSERGCPL PRAPRGRRRP NPPRKPVRPR421GPGRPRDKGV PVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
432 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.