Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNK2 / TREK-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114097-100
Catalog Number: LS-G114097
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNK2 (NM_014217), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HTREK-1c | K2p2.1 | KCNK2 | TREK-1 K(+) channel subunit | TREK1 | Two-pore potassium channel 1 | HTREK-1e | K2P2.1 potassium channel | TPKC1 | TREK-1
- Origin Species
- Human
- NCBI GenBank
- NM_001017424
- SwissProt
- O95069
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPDLLDPK SAAQNSKPRL SFSTKPTVLA SRVESDTTIN VMKWKTVSTI FLVVVLYLII61GATVFKALEQ PHEISQRTTI VIQKQTFISQ HSCVNSTELD ELIQQIVAAI NAGIIPLGNT121SNQISHWDLG SSFFFAGTVI TTIGFGNISP RTEGGKIFCI IYALLGIPLF GFLLAGVGDQ181LGTIFGKGIA KVEDTFIKWN VSQTKIRIIS TIIFILFGCV LFVALPAIIF KHIEGWSALD241AIYFVVITLT TIGFGDYVAG GSDIEYLDFY KPVVWFWILV GLAYFAAVLS MIGDWLRVIS301KKTKEEVGEF RAHAAEWTAN VTAEFKETRR RLSVEIYDKF QRATSIKRKL SAELAGNHNQ361ELTPCRRTLS VNHLTSERDV LPPLLKTESI YLNGLTPHCA GEEIAVIENI KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
411 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.