Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNK13 / THIK-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110491-100
Catalog Number: LS-G110491
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNK13 (NM_022054), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- K2p13.1 | K2P13.1 potassium channel | KCNK13 | THIK-1 | THIK1
- Origin Species
- Human
- NCBI GenBank
- NM_022054
- SwissProt
- Q9HB14
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGRGFSWGP GHLNEDNARF LLLAALIVLY LLGGAAVFSA LELAHERQAK QRWEERLANF61SRGHNLSRDE LRGFLRHYEE ATRAGIRVDN VRPRWDFTGA FYFVGTVVST IGFGMTTPAT121VGGKIFLIFY GLVGCSSTIL FFNLFLERLI TIIAYIMKSC HQRQLRRRGA LPQESLKDAG181QCEVDSLAGW KPSVYYVMLI LCTASILISC CASAMYTPIE GWSYFDSLYF CFVAFSTIGF241GDLVSSQNAH YESQGLYRFA NFVFILMGVC CIYSLFNVIS ILIKQSLNWI LRKMDSGCCP301QCQRGLLRSR RNVVMPGSVR NRCNISIETD GVAESDTDGR RLSGEMISMK DLLAANKASL361AILQKQLSEM ANGCPHQTST LARDNEFSGG VGAFAIMNNR LAETSGDRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
408 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.