Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNH7 / ERG3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108337-100
Catalog Number: LS-G108337
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNH7 (NM_033272), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Eag related protein 3 | ERG-3 | HERG3 | ERG3 | Kv11.3 | HERG-3 | Eag-related protein 3 | KCNH7
- Origin Species
- Human
- NCBI GenBank
- NM_033272
- SwissProt
- Q9NS40
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPVRRGHVAP QNTFLGTIIR KFEGQNKKFI IANARVQNCA IIYCNDGFCE MTGFSRPDVM61QKPCTCDFLH GPETKRHDIA QIAQALLGSE ERKVEVTYYH KNGSTFICNT HIIPVKNQEG121VAMMFIINFE YVTDNENAAT PERVNPILPI KTVNRKFFGF KFPGLRVLTY RKQSLPQEDP181DVVVIDSSKH SDDSVAMKHF KSPTKESCSP SEADDTKALI QPSKCSPLVN ISGPLDHSSP241KRQWDRLYPD MLQSSSQLSH SRSRESLCSI RRASSVHDIE GFGVHPKNIF RDRHASEDNG301RNVKGPFNHI KSSLLGSTSD SNLNKYSTIN KIPQLTLNFS EVKTEKKNSS PPSSDKTIIA361PKVKDRTHNV TEKVTQVLSL GADVLPEYKL QTPRINKFTI LHYSPFKAVW DWLILLLVIY421TAIFTPYSAA FLLNDREEQK RRECGYSCSP LNVVDLIVDI MFIIDILINF RTTYVNQNEE481VVSDPAKIAI HYFKGWFLID MVAAIPFDLL IFGSGSDETT TLIGLLKTAR LLRLVRVARK541LDRYSEYGAA VLMLLMCIFA LIAHWLACIW YAIGNVERPY LTDKIGWLDS LGQQIGKRYN601DSDSSSGPSI KDKYVTALYF TFSSLTSVGF GNVSPNTNSE KIFSICVMLI GSLMYASIFG661NVSAIIQRLY SGTARYHMQM LRVKEFIRFH QIPNPLRQRL EEYFQHAWTY TNGIDMNMVL721KGFPECLQAD ICLHLNQTLL QNCKAFRGAS KGCLRALAMK FKTTHAPPGD TLVHCGDVLT781ALYFLSRGSI EILKDDIVVA ILGKNDIFGE MVHLYAKPGK SNADVRALTY CDLHKIQRED841LLEVLDMYPE FSDHFLTNLE LTFNLRHESA KADLLRSQSM NDSEGDNCKL RRRKLSFESE901GEKENSTNDP EDSADTIRHY QSSKRHFEEK KSRSSSFISS IDDEQKPLFS GIVDSSPGIG961KASGLDFEET VPTSGRMHID KRSHSCKDIT DMRSWERENA HPQPEDSSPS ALQRAAWGIS1021ETESDLTYGE VEQRLDLLQE QLNRLESQMT TDIQTILQLL QKQTTVVPPA YSMVTAGSEY1081QRPIIQLMRT SQPEASIKTD RSFSPSSQCP EFLDLEKSKL KSKESLSSGV HLNTASEDNL1141TSLLKQDSDL SLELHLRQRK TYVHPIRHPS LPDSSLSTVG IVGLHRHVSD PGLPGKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1196 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.