Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNH6 / Kv11.2 / ERG2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105418-100
Catalog Number: LS-G105418
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNH6 (NM_173092), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Eag related protein 2 | Eag-related gene member 2 | ERG-2 | Eag-related protein 2 | ERG2 | HERG-2 | HERG2 | KCNH6 | Kv11.2
- Origin Species
- Human
- NCBI GenBank
- NM_030779
- SwissProt
- Q9H252
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPVRRGHVAP QNTYLDTIIR KFEGQSRKFL IANAQMENCA IIYCNDGFCE LFGYSRVEVM61QQPCTCDFLT GPNTPSSAVS RLAQALLGAE ECKVDILYYR KDASSFRCLV DVVPVKNEDG121AVIMFILNFE DLAQLLAKCS SRSLSQRLLS QSFLGSEGSH GRPGGPGPGT GRGKYRTISQ181IPQFTLNFVE FNLEKHRSSS TTEIEIIAPH KVVERTQNVT EKVTQVLSLG ADVLPEYKLQ241APRIHRWTIL HYSPFKAVWD WLILLLVIYT AVFTPYSAAF LLSDQDESRR GACSYTCSPL301TVVDLIVDIM FVVDIVINFR TTYVNTNDEV VSHPRRIAVH YFKGWFLIDM VAAIPFDLLI361FRTGSDETTT LIGLLKTARL LRLVRVARKL DRYSEYGAAV LFLLMCTFAL IAHWLACICS421LTSVGFGNVS PNTNSEKVFS ICVMLIGSLM YASIFGNVSA IIQRLYSGTA RYHTQMLRVK481EFIRFHQIPN PLRQRLEEYF QHAWSYTNGI DMNAVLKGFP ECLQADICLH LHRALLQHCP541AFSGAGKGCL RALAVKFKTT HAPPGDTLVH LGDVLSTLYF ISRGSIEILR DDVVVAILGK601NDIFGEPVSL HAQPGKSSAD VRALTYCDLH KIQRADLLEV LDMYPAFAES FWSKLEVTFN661LRDAAGGLHS SPRQAPGSQD HQGFFLSDNQ SDAAPPLSIS DASGLWPELL QEMPPRHSPQ721SPQEDPDCWP LKLGSRLEQL QAQMNRLESR VSSDLSRILQ LLQKPMPQGH ASYILEAPAS781NDLALVPIAS ETTSPGPRLP QGFLPPAQTP SYGDLDDCSP KHRNSSPRMP HLAVATDKTL841APSSEQEQPE GLWPPLASPL HPLEVQGLIC GPCFSSLPEH LGSVPKQLDF QRHGSDPGFA901GSWGHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
905 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.