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KCNH4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KCNH4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KCNH4 antibodies →
$725.00each
Catalog Number: LS-G114517-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNH4 (NM_012285), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BEC2 | ELK channel 1 | Kv12.3 | KCNH4
Origin Species
Human
NCBI GenBank
NM_012285
SwissProt
Q9UQ05

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVMKGLLAP QNTFLDTIAT RFDGTHSNFL LANAQGTRGF PIVYCSDGFC ELTGYGRTEV
61MQKTCSCRFL YGPETSEPAL QRLHKALEGH QEHRAEICFY RKDGSAFWCL LDMMPIKNEM
121GEVVLFLFSF KDITQSGSPG LGPQGGRGDS NHENSLGRRG ATWKFRSARR RSRTVLHRLT
181GHFGRRGQGG MKANNNVFEP KPSVPEYKVA SVGGSRCLLL HYSVSKAIWD GLILLATFYV
241AVTVPYNVCF SGDDDTPITS RHTLVSDIAV EMLFILDIIL NFRTTYVSQS GQVISAPRSI
301GLHYLATWFF IDLIAALPFD LLYIFNITVT SLVHLLKTVR LLRLLRLLQK LERYSQCSAV
361VLTLLMSVFA LLAHWMACIW YVIGRREMEA NDPLLWDIGW LHELGKRLEV PYVNGSVGGP
421SRRSAYIAAL YFTLSSLTSV GFGNVCANTD AEKIFSICTM LIGALMHAVV FGNVTAIIQR
481MYSRRSLYHS RMKDLKDFIR VHRLPRPLKQ RMLEYFQTTW AVNSGIDANE LLRDFPDELR
541ADIAMHLNRE ILQLPLFGAA SRGCLRALSL HIKTSFCAPG EYLLRRGDAL QAHYYVCSGS
601LEVLRDNMVL AILGKGDLIG ADIPEPGQEP GLGADPNFVL KTSADVKALT YCGLQQLSSR
661GLAEVLRLYP EYGAAFRAGL PRDLTFNLRQ GSDTSGLSRF SRSPRLSQPR SESLGSSSDK
721TLPSITEAES GAEPGGGPRP RRPLLLPNLS PARPRGSLVS LLGEELPPFS ALVSSPSLSP
781SLSPALAGQG HSASPHGPPR CSAAWKPPQL LIPPLGTFGP PDLSPRIVDG IEDSGSTAEA
841PSFRFSRRPE LPRPRSQAPP TGTRPSPELA SEAEEVKEKV CRLNQEISRL NQEVSQLSRE
901LRHIMGLLQA RLGPPGHPAG SAWTPDPPCP QLRPPCLSPC ASRPPPSLQD TTLAEVHCPA
961SVGTMETGTA LLDLRPSILP PYPSEPDPLG PSPVPEASPP TPSLLRHSFQ SRSDTFH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1017 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.