Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNH3 / Kv12.2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114516-100
Catalog Number: LS-G114516
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNH3 (NM_012284), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BEC1 | ELK channel 2 | Ether-a-go-go-like | KCNH3 | KIAA1282 | ELK2 | Kv12.2 | Melk2
- Origin Species
- Human
- NCBI GenBank
- AB033108
- SwissProt
- Q9ULD8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPAMRGLLAP QNTFLDTIAT RFDGTHSNFV LGNAQVAGLF PVVYCSDGFC DLTGFSRAEV61MQRGCACSFL YGPDTSELVR QQIRKALDEH KEFKAELILY RKSGLPFWCL LDVIPIKNEK121GEVALFLVSH KDISETKNRG GPDRWKETGG GRRRYGRARS KGFNANRRRS RAVLYHLSGH181LQKQPKGKHK LNKGVFGEKP NLPEYKVAAI RKSPFILLHC GALRATWDGF ILLATLYVAV241TVPYSVCVST AREPSAARGP PSVCDLAVEV LFILDIVLNF RTTFVSKSGQ VVFAPKSICL301HYVTTWFLLD VIAALPFDLL HAFKVNVYFG AHLLKTVRLL RLLRLLPRLD RYSQYSAVVL361TLLMAVFALL AHWVACVWFY IGQREIESSE SELPEIGWLQ ELARRLETPY YLVGRRPAGG421NSSGQSDNCS SSSEANGTGL ELLGGPSLRS AYITSLYFAL SSLTSVGFGN VSANTDTEKI481FSICTMLIGA LMHAVVFGNV TAIIQRMYAR RFLYHSRTRD LRDYIRIHRI PKPLKQRMLE541YFQATWAVNN GIDTTELLQS LPDELRADIA MHLHKEVLQL PLFEAASRGC LRALSLALRP601AFCTPGEYLI HQGDALQALY FVCSGSMEVL KGGTVLAILG KGDLIGCELP RREQVVKANA661DVKGLTYCVL QCLQLAGLHD SLALYPEFAP RFSRGLRGEL SYNLGAGGGS AEVDTSSLSG721DNTLMSTLEE KETDGEQGPT VSPAPADEPS SPLLSPGCTS SSSAAKLLSP RRTAPRPRLG781GRGRPGRAGA LKAEAGPSAP PRALEGLRLP PMPWNVPPDL SPRVVDGIED GCGSDQPKFS841FRVGQSGPEC SSSPSPGPES GLLTVPHGPS EARNTDTLDK LRQAVTELSE QVLQMREGLQ901SLRQAVQLVL APHREGPCPR ASGEGPCPAS TSGLLQPLCV DTGASSYCLQ PPAGSVLSGT961WPHPRPGPPP LMAPWPWGPP ASQSSPWPRA TAFWTSTSDS EPPASGDLCS EPSTPASPPP1021SEEGARTGPA EPVSQAEATS TGEPPPGSGG LALPWDPHSL EMVLIGCHGS GTVQWTQEEG1081TGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1083 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.