Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNF1 / KCNF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118110-100
Catalog Number: LS-G118110
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNF1 (NM_002236), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IK8 | KH1 | KV5.1 | Potassium channel Kv5.1 | Potassium channel KH1 | KCNF | KCNF1
- Origin Species
- Human
- NCBI GenBank
- NM_002236
- SwissProt
- Q9H3M0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGSGERSLP EPGSQSSAAS DDIEIVVNVG GVRQVLYGDL LSQYPETRLA ELINCLAGGY61DTIFSLCDDY DPGKREFYFD RDPDAFKCVI EVYYFGEVHM KKGICPICFK NEMDFWKVDL121KFLDDCCKSH LSEKREELEE IARRVQLILD DLGVDAAEGR WRRCQKCVWK FLEKPESSCP181ARVVAVLSFL LILVSSVVMC MGTIPELQVL DAEGNRVEHP TLENVETACI GWFTLEYLLR241LFSSPNKLHF ALSFMNIVDV LAILPFYVSL TLTHLGARMM ELTNVQQAVQ ALRIMRIARI301FKLARHSSGL QTLTYALKRS FKELGLLLMY LAVGIFVFSA LGYTMEQSHP ETLFKSIPQS361FWWAIITMTT VGYGDIYPKT TLGKLNAAIS FLCGVIAIAL PIHPIINNFV RYYNKQRVLE421TAAKHELELM ELNSSSGGEG KTGGSRSDLD NLPPEPAGKE APSCSSRLKL SHSDTFIPLL481TEEKHHRTRL QSCKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
494 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.