Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCND1 / Kv4.1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100431-100
Catalog Number: LS-G100431
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCND1 (NM_004979), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KCND1 | MShal1 | Shal-type potassium channel | KV4.1
- Origin Species
- Human
- NCBI GenBank
- NM_004979
- SwissProt
- Q9NSA2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAGLATWLP FARAAAVGWL PLAQQPLPPA PGVKASRGDE VLVVNVSGRR FETWKNTLDR61YPDTLLGSSE KEFFYDADSG EYFFDRDPDM FRHVLNFYRT GRLHCPRQEC IQAFDEELAF121YGLVPELVGD CCLEEYRDRK KENAERLAED EEAEQAGDGP ALPAGSSLRQ RLWRAFENPH181TSTAALVFYY VTGFFIAVSV IANVVETIPC RGSARRSSRE QPCGERFPQA FFCMDTACVL241IFTGEYLLRL FAAPSRCRFL RSVMSLIDVV AILPYYIGLL VPKNDDVSGA FVTLRVFRVF301RIFKFSRHSQ GLRILGYTLK SCASELGFLL FSLTMAIIIF ATVMFYAEKG TNKTNFTSIP361AAFWYTIVTM TTLGYGDMVP STIAGKIFGS ICSLSGVLVI ALPVPVIVSN FSRIYHQNQR421ADKRRAQQKV RLARIRLAKS GTTNAFLQYK QNGGLEDSGS GEEQALCVRN RSAFEQQHHH481LLHCLEKTTC HEFTDELTFS EALGAVSPGG RTSRSTSVSS QPVGPGSLLS SCCPRRAKRR541AIRLANSTAS VSRGSMQELD MLAGLRRSHA PQSRSSLNAK PHDSLDLNCD SRDFVAAIIS601IPTPPANTPD ESQPSSPGGG GRAGSTLRNS SLGTPCLFPE TVKISSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
647 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.