Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNC4 / Kv3.4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100433-100
Catalog Number: LS-G100433
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNC4 (NM_004978), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf30 | HKSHIIIC | K+ channel subunit | KSHIIIC | KV3.4 | KCNC4
- Origin Species
- Human
- NCBI GenBank
- NM_004978
- SwissProt
- Q03721
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MISSVCVSSY RGRKSGNKPP SKTCLKEEMA KGEASEKIII NVGGTRHETY RSTLRTLPGT61RLAWLADPDG GGRPETDGGG VGSSGSSGGG GCEFFFDRHP GVFAYVLNYY RTGKLHCPAD121VCGPLFEEEL TFWGIDETDV EPCCWMTYRQ HRDAEEALDI FESPDGGGSG AGPSDEAGDD181ERELALQRLG PHEGGAGHGA GSGGCRGWQP RMWALFEDPY SSRAARVVAF ASLFFILVSI241TTFCLETHEA FNIDRNVTEI LRVGNITSVH FRREVETEPI LTYIEGVCVL WFTLEFLVRI301VCCPDTLDFV KNLLNIIDFV AILPFYLEVG LSGLSSKAAR DVLGFLRVVR FVRILRIFKL361TRHFVGLRVL GHTLRASTNE FLLLIIFLAL GVLIFATMIY YAERIGARPS DPRGNDHTDF421KNIPIGFWWA VVTMTTLGYG DMYPKTWSGM LVGALCALAG VLTIAMPVPV IVNNFGMYYS481LAMAKQKLPK KRKKHVPRPA QLESPMYCKS EETSPRDSTC SDTSPPAREE GMIERKRADS541KQNGDANAVL SDEEGAGLTQ PLASSPTPEE RRALRRSTTR DRNKKAAACF LLSTGDYACA601DGSVRKGTFV LRDLPLQHSP EAACPPTAGT LFLPHSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
635 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.