Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNA7 / Kv1.7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102009-100
Catalog Number: LS-G102009
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNA7 (NM_031886), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HAK6 | KCNA7 | RK6 | KV1.7
- Origin Species
- Human
- NCBI GenBank
- NM_031886
- SwissProt
- Q96RP8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPRCPPPCG CCERLVLNVA GLRFETRART LGRFPDTLLG DPARRGRFYD DARREYFFDR61HRPSFDAVLY YYQSGGRLRR PAHVPLDVFL EEVAFYGLGA AALARLREDE GCPVPPERPL121PRRAFARQLW LLFEFPESSQ AARVLAVVSV LVILVSIVVF CLETLPDFRD DRDGTGLAAA181AAAGPFPAPL NGSSQMPGNP PRLPFNDPFF VVETLCICWF SFELLVRLLV CPSKAIFFKN241VMNLIDFVAI LPYFVALGTE LARQRGVGQQ AMSLAILRVI RLVRVFRIFK LSRHSKGLQI301LGQTLRASMR ELGLLIFFLF IGVVLFSSAV YFAEVDRVDS HFTSIPESFW WAVVTMTTVG361YGDMAPVTVG GKIVGSLCAI AGVLTISLPV PVIVSNFSYF YHRETEGEEA GMFSHVDMQP421CGPLEGKANG GLVDGEVPEL PPPLWAPPGK HLVTEVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.