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KBTBD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KBTBD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KBTBD7 antibodies →
$504.00each
Catalog Number: LS-G109024-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KBTBD7 (NM_032138), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KBTBD7 | DKFZP434E2318
Origin Species
Human
NCBI GenBank
NM_032138
SwissProt
Q8WVZ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQSREDVPRS RRLASPRGGR RPKRISKPSV SAFFTGPEEL KDTAHSAALL AQLKSFYDAR
61LLCDVTIEVV TPGSGPGTGR LFSCNRNVLA AACPYFKSMF TGGMYESQQA SVTMHDVDAE
121SFEVLVDYCY TGRVSLSEAN VQRLYAASDM LQLEYVREAC ASFLARRLDL TNCTAILKFA
181DAFDHHKLRS QAQSYIAHNF KQLSRMGSIR EETLADLTLA QLLAVLRLDS LDIESERTVC
241HVAVQWLEAA AKERGPSAAE VFKCVRWMHF TEEDQDYLEG LLTKPIVKKY CLDVIEGALQ
301MRYGDLLYKS LVPVPNSSSS SSSSNSLVSA AENPPQRLGM CAKEMVIFFG HPRDPFLCYD
361PYSGDIYTMP SPLTSFAHTK TVTSSAVCVS PDHDIYLAAQ PRKDLWVYKP AQNSWQQLAD
421RLLCREGMDV AYLNGYIYIL GGRDPITGVK LKEVECYSVQ RNQWALVAPV PHSFYSFELI
481VVQNYLYAVN SKRMLCYDPS HNMWLNCASL KRSDFQEACV FNDEIYCICD IPVMKVYNPA
541RGEWRRISNI PLDSETHNYQ IVNHDQKLLL ITSTTPQWKK NRVTVYEYDT REDQWINIGT
601MLGLLQFDSG FICLCARVYP SCLEPGQSFI TEEDDARSES STEWDLDGFS ELDSESGSSS
661SFSDDEVWVQ VAPQRNAQDQ QGSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.