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KBTBD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KBTBD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112616-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KBTBD4 (NM_016506), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BKLHD4 | HSPC252 | KBTBD4
Origin Species
Human
NCBI GenBank
NM_018095

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESPEEPGAS MDENYFVNYT FKDRSHSGRV AQGIMKLCLE EELFADVTIS VEGREFQLHR
61LVLSAQSCFF RSMFTSNLKE AHNRVIVLQD VSESVFQLLV DYIYHGTVKL RAEELQEIYE
121VSDMYQLTSL FEECSRFLAR TVQVGNCLQV MWLADRHSDP ELYTAAKHCA KTHLAQLQNT
181EEFLHLPHRL LTDIISDGVP CSQNPTEAIE AWINFNKEER EAFAESLRTS LKEIGENVHI
241YLIGKESSRT HSLAVSLHCA EDDSISVSGQ NSLCHQITAA CKHGGDLYVV GGSIPRRMWK
301CNNATVDWEW CAPLPRDRLQ HTLVSVPGKD AIYSLGGKTL QDTLSNAVIY YRVGDNVWTE
361TTQLEVAVSG AAGANLNGII YLLGGEENDL DFFTKPSRLI QCFDTETDKC HVKPYVLPFA
421GRMHAAVHKD LVFIVAEGDS LVCYNPLLDS FTRLCLPEAW SSAPSLWKIA SCNGSIYVFR
481DRYKKGDANT YKLDPATSAV TVTRGIKVLL TNLQFVLA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.