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KBTBD12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KBTBD12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KBTBD12 antibodies →
$725.00each
Catalog Number: LS-G102754-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KBTBD12 (NM_207335), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KLHDC6 | KBTBD12 | Kelch domain containing 6
Origin Species
Human
NCBI GenBank
NM_207335
SwissProt
Q3ZCT8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MECKIEGKEK YQHSLNLLNK IQNMKELAEM IDVVLTAEGE KFPCHRLVLA AFSPYFKAMF
61TCGLLECNQR EVILYDITAE SVSVLLNYMY NAALEINNAN VQTVAMAAYF MQMEEVFSVC
121QKYMMDHMDA SNCLGIYYFA KQIGAEDLSD RSKKYLYQHF AEVSLHEEIL EIEVHQFLTL
181IKSDDLNISR EESILDLVLR WVNHNKELRT VHLVELLKQV RLELVNPSFL RQALRRNTML
241LCDADCVDII QNAFKAIKTP QQHSLNLRYG METTSLLLCI GNNSSGIRSR HRSYGDASFC
301YDPVSRKTYF ISSPKYGEGL GTVCTGVVME NNTIIVAGEA SASKLSRQKN KNVEIYRYHD
361RGNQFWEKLC TAEFRELYAL GSIHNDLYVI GGQMKIKNQY LITNCVDKYS VERDNWKRVS
421PLPLQLACHA VVTVNNKLYV IGGWTPQMDL PDEEPDRLSN KLLQYDPSQD QWSVRAPMKY
481SKYRFSTAVV NSEIYVLGGI GCVGQDKGQV RKCLDVVEIY NPDGDFWREG PPMPSPLLSL
541RTNSTNAGAV DGKLYVCGGF HGADRHEVIS KEILELDPWE NQWNVVAINV LMHDSYDVCL
601VARMNPRDLI PPPSDLVEEG NEH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.