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KBTBD11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KBTBD11 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KBTBD11 antibodies →
$725.00each
Catalog Number: LS-G113642-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KBTBD11 (NM_014867), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CMLAP | KIAA0711 | KBTBD11 | KLHDC7C
Origin Species
Human
NCBI GenBank
AB018254
SwissProt
O94819

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEHAVAPCVL YPGTEPGAAG ESESEGAASP AQTPCSLGAS LCFSSGEESP PQSLASAAEG
61AATSPPSSGG PRVVERQWEA GSAGAASPEE LASPEERACP EEPAAPSPEP RVWLEDPASP
121EEPGEPAPVP PGFGAVYGEP DLVLEVSGRR LRAHKAVLAA RSDYFRARAS RDVLRVQGVS
181LTALRLLLAD AYSGRMAGVR PDNVAEVVAG ARRLQLPGAA QRATDAVGPQ LSLANCYEVL
241SAAKRQRLNE LRDAAYCFMS DHYLEVLREP AVFGRLSGAE RDLLLRRRLR AGRAHLLAAA
301LGPAGERAGS RPQSPSGDAD ARGDAAVYCF HAAAGEWREL TRLPEGAPAR GCGLCVLYNY
361LFVAGGVAPA GPDGRARPSD QVFCYNPATD SWSAVRPLRQ ARSQLRLLAL DGHLYAVGGE
421CLLSVERYDP RADRWAPVAP LPRGAFAVAH EATTCHGEIY VSGGSLFYRL LKYDPRRDEW
481QECPCSSSRE RSADMVALDG FIYRFDLSGS RGEAQAAGPS GVSVSRYHCL AKQWSPCVAP
541LRLPGGPTGL QPFRCAALDG AIYCVSRAGT WRFQPAREGE AGGDAGQGGG FEALGAPLDV
601RGVLIPFALS LPEKPPRGEQ GAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.