LSBio
KAZN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KAZN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KAZN antibodies →
$725.00each
Catalog Number: LS-G103262-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kazrin(KAZN) (NM_201628), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kazrin | KAZN | KIAA1026 | KAZ | RP1-21O18.1
Origin Species
Human
NCBI GenBank
AB028949
SwissProt
Q674X7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMEDNKQLAL RIDGAVQSAS QEVTNLRAEL TATNRRLAEL SGGGGPGPGP GAAASASAAG
61DSAATNMENP QLGAQVLLRE EVSRLQEEVH LLRQMKEMLA KDLEESQGGK SSEVLSATEL
121RVQLAQKEQE LARAKEALQA MKADRKRLKG EKTDLVSQMQ QLYATLESRE EQLRDFIRNY
181EQHRKESEDA VKALAKEKDL LEREKWELRR QAKEATDHAT ALRSQLDLKD NRMKELEAEL
241AMAKQSLATL TKDVPKRHSL AMPGETVLNG NQEWVVQADL PLTAAIRQSQ QTLYHSHPPH
301PADRQAVRVS PCHSRQPSVI SDASAAEGDR SSTPSDINSP RHRTHSLCNG DSPGPVQKNL
361HNPIVQSLED LEDQKRKKKK EKMGFGSISR VFARGKQRKS LDPGLFDDSD SQCSPTRQSL
421SLSEGEEQMD RLQQVELVRT TPMSHWKAGT VQAWLEVVMA MPMYVKACTE NVKSGKVLLS
481LSDEDLQLGL GVCSSLHRRK LRLAIEDYRD AEAGRSLSKA AELDHHWVAK AWLNDIGLSQ
541YSQAFQNHLV DGRMLNSLMK RDLEKHLNVS KKFHQVSILL GIELLYQVNF SREALQERRA
601RCETQNIDPV VWTNQRVLKW VRDIDLKEYA DNLTNSGVHG AVLVLEPTFN AEAMATALGI
661PSGKHILRRH LAEEMSAVFH PANSTGIREA ERFGTPPGRA SSVTRAGKEE NSSGLKYKAG
721RLPLGKIGRG FSSKDPDFHD DYGSLQNEDC GDDDPQSRLE QCRLEGYNSL EVTNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

775 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.