LSBio
KATNB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KATNB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KATNB1 antibodies →
$504.00each
Catalog Number: LS-G115667-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human katanin p80(KATNB1) (NM_005886), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KAT | Katanin p80 subunit B1 | KATNB1 | Katanin (80 kDa) | p80 katanin
Origin Species
Human
NCBI GenBank
NM_005886
SwissProt
Q9BVA0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATPVVTKTA WKLQEIVAHA SNVSSLVLGK ASGRLLATGG DDCRVNLWSI NKPNCIMSLT
61GHTSPVESVR LNTPEELIVA GSQSGSIRVW DLEAAKILRT LMGHKANICS LDFHPYGEFV
121ASGSQDTNIK LWDIRRKGCV FRYRGHSQAV RCLRFSPDGK WLASAADDHT VKLWDLTAGK
181MMSEFPGHTG PVNVVEFHPN EYLLASGSSD RTIRFWDLEK FQVVSCIEGE PGPVRSVLFN
241PDGCCLYSGC QDSLRVYGWE PERCFDVVLV NWGKVADLAI CNDQLIGVAF SQSNVSSYVV
301DLTRVTRTGT VARDPVQDHR PLAQPLPNPS APLRRIYERP STTCSKPQRV KQNSESERRS
361PSSEDDRDER ESRAEIQNAE DYNEIFQPKN SISRTPPRRS EPFPAPPEDD AATAKEAAKP
421SPAMDVQFPV PNLEVLPRPP VVASTPAPKA EPAIIPATRN EPIGLKASDF LPAVKIPQQA
481ELVDEDAMSQ IRKGHDTMCV VLTSRHKNLD TVRAVWTMGD IKTSVDSAVA INDLSVVVDL
541LNIVNQKASL WKLDLCTTVL PQIEKLLQSK YESYVQTGCT SLKLILQRFL PLITDMLAAP
601PSVGVDISRE ERLHKCRLCY KQLKSISGLV KSKSGLSGRH GSTFRELHLL MASLDSGPTR
661TRRLEQKLIS EEDLAANDIL DYKDDDDKV

689 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.