Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KAT5 / TIP60 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104187-100
Catalog Number: LS-G104187
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KAT5(KAT5) (NM_182710), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 60 kDa Tat-interactive protein | CPLA(2)-interacting protein | CPLA2 interacting protein | HIV-1 Tat interactive protein | ESA1 | HTATIP1 | Histone acetyltransferase KAT5 | KAT5 | K-acetyltransferase 5 | Lysine acetyltransferase 5 | HTATIP | K(lysine) acetyltransferase 5 | PLIP | TIP60 | Tat interacting protein | 60kDa | TIP | ZC2HC5
- Origin Species
- Human
- NCBI GenBank
- NM_006388
- SwissProt
- Q92993
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEVVSPVPG AGRREPGEVG RARGPPVADP GVALSPQGEI IEGCRLPVLR RNQDNEDEWP61LAEILSVKDI SGRKLFYVHY IDFNKRLDEW VTHERLDLKK IQFPKKEAKT PTKNGLPGSR121PGSPEREVPA SAQASGKTLP IPVQITLRFN LPKEREAIPG GEPDQPLSSS SCLQPNHRST181KRKVEVVSPA TPVPSETAPA SVFPQNGAAR RAVAAQPGRK RKSNCLGTDE DSQDSSDGIP241SAPRMTGSLV SDRSHDDIVT RMKNIECIEL GRHRLKPWYF SPYPQELTTL PVLYLCEFCL301KYGRSLKCLQ RHLTKCDLRH PPGNEIYRKG TISFFEIDGR KNKSYSQNLC LLAKCFLDHK361TLYYDTDPFL FYVMTEYDCK GFHIVGYFSK EKESTEDYNV ACILTLPPYQ RRGYGKLLIE421FSYELSKVEG KTGTPEKPLS DLGLLSYRSY WSQTILEILM GLKSESGERP QITINEISEI481TSIKKEDVIS TLQYLNLINY YKGQYILTLS EDIVDGHERA MLKRLLRIDS KCLHFTPKDW541SKRGKWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
546 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.