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KANK4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KANK4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104418-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KANK4 (NM_181712), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD38 | DJ1078M7.1 | RP5-1155K23.5 | Ankyrin repeat domain 38 | KANK4 | KIAA0172
Origin Species
Human
NCBI GenBank
NM_181712
SwissProt
Q5T7N3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKTDAKDQS SQGDEEKDPP KSHPYSVETP YGFHLDLDFL KYVDDIEKGN TIKRIPIHRR
61AKQAKFSTLP RNFSLPDSGA RPPAAPPLQN WSPVVPREAS LGTQEQNQSP PLGNAPQAST
121SRSEVSYHRK ALLAEATRQL EAAEPEDAEL TFGSGRPQLL RASSMPATLL HSRASEEPGL
181SLGPPAPPAL PPLQGEGSVC DGTFEPAEGL AGFHSSSPRA STRIPELVQE GAEPPEGVVK
241VPNHLPLPGP PFSFQNVLVV LEDKEDEHNA REAEVLFTPG SPTPSPPPLP SPIPENELLL
301EEIELNISEI PPPPPVEVDM RSIGIRVTEE SLGLARVDPG SISSLKQQVS ALEGELSGRT
361EELAQVRTAL QQQEEEIKAR EQRIRELEFT VAQLEGQFHQ ENAKDTQGQT DVMVNTDPVH
421GLLTRESCDK GIEVNLLGSM ESESWGHRGE ENGLLWGPDG HKQGNQSPAE RVLLPQLSLP
481QGPEQVLTSS VHSFLSTELR IEEAGTEQEG GPQGGTRGAG GFLWGSDRKT PPAGREETSS
541NLPGKEHPGR PPSSPTDATI GQYVKKIQEL LQEQWNCLEH GYPELASAIK QPASKLSSIQ
601SQLLSSLNLL LSAYSAQAHP PKEPPASSSS PPVEISPSTS LKSIMKKKDY GFRAGGNGTK
661KNLQFVGVNG GYETTSSEET SGEDSTPEDL SDSEAEKKCD GPDHKHVKDA HLTCEAGQGI
721PEGTCHAAQE SGPGEEVPHS KAERYKPSEE FLNACRALSQ HLPETGTTTD QLLRQSLNTI
781SQEWFRVSSR KSSSPAVVAS YLHEVQPHSP HFLKLLVNLA DRNGNTALHY SVSHSNFSIA
841KLLLETGVCN VDHQNKAGYT AVMITPLASA ETNEDMAVVW KLLREGNVNI QATQGGQTAL
901MLGVSHDRED MVQALLSCQA DVNLQDHDGS SALMLACHHG NVDLVRLLLA HPACDSSLTD
961KAGRTALSIA LKSPTHMEIA GLLRAHAEQG RSLGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

995 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.