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KANK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KANK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KANK3 antibodies →
$504.00each
Catalog Number: LS-G102563-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KANK3 (NM_198471), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD47 | Ankyrin repeat domain 47 | KANK3
Origin Species
Human
NCBI GenBank
AA622394
SwissProt
Q6NY19

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKFALNQNL PDLGGPRLCP VPAAGGARSP SSPYSVETPY GFHLDLDFLK YIEELERGPA
61ARRAPGPPTS RRPRAPRPGL AGARSPGAWT SSESLASDDG GAPGILSQGA PSGLLMQPLS
121PRAPVRNPRV EHTLRETSRR LELAQTHERA PSPGRGVPRS PRGSGRSSPA PNLAPASPGP
181AQLQLVREQM AAALRRLREL EDQARTLPEL QEQVRALRAE KARLLAGRAQ PEPDGEAETR
241PDKLAQLRRL TERLATSERG GRARASPRAD SPDGLAAGRS EGALQVLDGE VGSLDGTPQT
301REVAAEAVPE TREAGAQAVP ETREAGVEAA PETVEADAWV TEALLGLPAA AERELELLRA
361SLEHQRGVSE LLRGRLRELE EAREAAEEAA AGARAQLREA TTQTPWSCAE KAAQTESPAE
421APSLTQESSP GSMDGDRAVA PAGILKSIMK KRDGTPGAQP SSGPKSLQFV GVLNGEYESS
481SSEDASDSDG DSENGGAEPP GSSSGSGDDS GGGSDSGTPG PPSGGDIRDP EPEAEAEPQQ
541VAQGRCELSP RLREACVALQ RQLSRPRGVA SDGGAVRLVA QEWFRVSSQR RSQAEPVARM
601LEGVRRLGPE LLAHVVNLAD GNGNTALHYS VSHGNLAIAS LLLDTGACEV NRQNRAGYSA
661LMLAALTSVR QEEEDMAVVQ RLFCMGDVNA KASQTGQTAL MLAISHGRQD MVATLLACGA
721DVNAQDADGA TALMCASEYG RLDTVRLLLT QPGCDPAILD NEGTSALAIA LEAEQDEVAA
781LLHAHLSSGQ PDTQSESPPG SQTATPGEGE CGDNGENPQV Q
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

821 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.