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KANK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KANK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KANK1 antibodies →
$504.00each
Catalog Number: LS-G105859-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD15(KANK1) (NM_153186), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD15 | CPSQ2 | KANK1 | KANK | Ankyrin repeat domain 15 | KIAA0172
Origin Species
Human
NCBI GenBank
D79994
SwissProt
Q14678

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1METRRRLEQE RATMQMTPGE FRRPRLASFG GMGTTSSLPS FVGSGNHNPA KHQLQNGYQG
61NGDYGSYAPA APTTSSMGSS IRHSPLSSGI STPVTNVSPM HLQHIREQMA IALKRLKELE
121EQVRTIPVLQ VKISVLQEEK RQLVSQLKNQ RAASQINVCG VRRRSYSAGN ASQLEQLSRA
181RRSGGELYID YEEEEMETVE QSTQRIKEFR QLTADMQALE QKIQDSSCEA SSELRENGEC
241RSVAVGAEEN MNDIVVYHRG SRSCKDAAVG TLVQMRNCGV SVTEAMLGVM TEADKEIELQ
301QQTIESLKEK IYGLEVQLRE TTHDREMTKL KQELQAAGSR KKVDKATMAQ PLVFSKVVEA
361VVQTRDQMVG SHMDLVDTCV GTSVETNSVG ISCQPECKNK VVGPELPMNW WIVKERVEMH
421DRCAGRSVEM CDKSVSVEVS VCETGSNTEE SVNDLTLLKT NLNLKEVRSI GCGDCSVDVT
481VCSPKECASR GVNTEAVSQV EAAVMAVPRT ADQDTSTDLE QVHQFTNTET ATLIESCTNT
541CLSTLDKQTS TQTVETRTVA VGEGRVKDIN SSTKTRSIGV GTLLSGHSGF DRPSAVKTKE
601SGVGQININD NYLVGLKMRT IACGPPQLTV GLTASRRSVG VGDDPVGESL ENPQPQAPLG
661MMTGLDHYIE RIQKLLAEQQ TLLAENYSEL AEAFGEPHSQ MGSLNSQLIS TLSSINSVMK
721SASTEELRNP DFQKTSLGKI TGNYLGYTCK CGGLQSGSPL SSQTSQPEQE VGTSEGKPIS
781SLDAFPTQEG TLSPVNLTDD QIAAGLYACT NNESTLKSIM KKKDGNKDSN GAKKNLQFVG
841INGGYETTSS DDSSSDESSS SESDDECDVI EYPLEEEEEE EDEDTRGMAE GHHAVNIEGL
901KSARVEDEMQ VQECEPEKVE IRERYELSEK MLSACNLLKN TINDPKALTS KDMRFCLNTL
961QHEWFRVSSQ KSAIPAMVGD YIAAFEAISP DVLRYVINLA DGNGNTALHY SVSHSNFEIV
1021KLLLDADVCN VDHQNKAGYT PIMLAALAAV EAEKDMRIVE ELFGCGDVNA KASQAGQTAL
1081MLAVSHGRID MVKGLLACGA DVNIQDDEGS TALMCASEHG HVEIVKLLLA QPGCNGHLED
1141NDGSTALSIA LEAGHKDIAV LLYAHVNFAK AQSPGTPRLG RKTSPGPTHR GSFD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1194 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.