Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KALRN / KALIRIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114892-100
Catalog Number: LS-G114892
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KALRN (NM_007064), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF24 | DUO | CHDS5 | DUET | HAPIP | Kalirin | RhoGEF kinase | Protein Duo | KALRN | TRAD
- Origin Species
- Human
- NCBI GenBank
- NM_001024660
- SwissProt
- O60229
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 144.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKGGDRAYTR GPSLGWLFAK CCCCFPCRDA YSHSSSENGG KSESVANLQA QPSLNSIHSS61PGPKRSTNTL KKWLTSPVRR LNSGKADGNI KKQKKVRDGR KSFDLGSPKP GDETTPQGDS121ADEKSKKGWG EDEPDEESHT PLPPPMKIFD NDPTQDEMSS SLLAARQAST EVPTAADLVN181AIEKLVKNKL SLEGSSYRGS LKDPAGCLNE GMAPPTPPKN PEEEQKAKAL RGRMFVLNEL241VQTEKDYVKD LGIVVEGFMK RIEEKGVPED MRGKDKIVFG NIHQIYDWHK DFFLAELEKC301IQEQDRLAQL FIKHERKLHI YVWYCQNKPR SEYIVAEYDA YFEEVKQEIN QRLTLSDFLI361KPIQRITKYQ LLLKDFLRYS EKAGLECSDI EKAVELMCLV PKRCNDMMNL GRLQGFEGTL421TAQGKLLQQD TFYVIELDAG MQSRTKERRV FLFEQIVIFS ELLRKGSLTP GYMFKRSIKM481NYLVLEENVD NDPCKFALMN RETSERVVLQ AANADIQQAW VQDINQVLET QRDFLNALQS541PIEYQRKERS TAVMRSQPAR LPQASPRPYS SVPAGSEKPP KGSSYNPPLP PLKISTSNGS601PGFEYHQPGD KFEASKQNDL GGCNGTSSMA VIKDYYALKE NEICVSQGEV VQVLAVNQQN661MCLVYQPASD HSPAAEGWVP GSILAPLTKA TAAESSDGSI KKSCSWHTLR MRKRAEVENT721GKNEATGPRK PKDILGNKVS VKETNSSEES ECDDLDPNTS MEILNPNFIQ EVAPEFLVPL781VDVTCLLGDT VILQCKVCGR PKPTITWKGP DQNILDTDNS SATYTVSSCD SGEITLKICN841LMPQDSGIYT CIATNDHGTT STSATVKVQG VPAAPNRPIA QERSCTSVIL RWLPPSSTGN901CTISGYTVEY REEGSQIWQQ SVASTLDTYL VIEDLSPGCP YQFRVSASNP WGISLPSEPS961EFVRLPEYDA AADGATISWK ENFDSAYTEL NEIGRGRFSI VKKCIHKATR KDVAVKFVSK1021KMKKKEQAAH EAALLQHLQH PQYITLHDTY ESPTSYILIL ELMDDGRLLD YLMNHDELME1081EKVAFYIRDI MEALQYLHNC RVAHLDIKPE NLLIDLRIPV PRVKLIDLED AVQISGHFHI1141HHLLGNPEFA APEVIQGIPV SLGTDIWSIG VLTYVMLSGV SPFLDESKEE TCINVCRVDF1201SFPHEYFCGV SNAARDFINV ILQEDFRRRP TAATCLQHPW LQPHNGSYSK IPLDTSRLAC1261FIERRKHQND VRPIPNVKSY IVNRVNQGTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1289 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.