Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
K13 / KCNG Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118111-100
Catalog Number: LS-G118111
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNG1 (NM_002237), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KCNG1 | K13 | KH2 | KV6.1 | KCNG | Potassium channel Kv6.1 | Potassium channel KH2
- Origin Species
- Human
- NCBI GenBank
- NM_002237
- SwissProt
- Q9UIX4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLLPGDNSD YDYSALSCTS DASFHPAFLP QRQAIKGAFY RRAQRLRPQD EPRQGCQPED61RRRRIIINVG GIKYSLPWTT LDEFPLTRLG QLKACTNFGD ILNVCDDYDV TCNEFFFDRN121PGAFGTILTF LRAGKLRLLR EMCALSFQEE LLYWGIAEDH LDGCCKRRYL QKIEEFAEMV181EREEEDDALD SEGRDSEGPA EGEGRLGRCM RRLRDMVERP HSGLPGKVFA CLSVLFVTVT241AVNLSVSTLP SLREEEEQGH CSQMCHNVFI VESVCVGWFS LEFLLRLIQA PSKFAFLRSP301LTLIDLVAIL PYYITLLVDG AAAGRRKPGA GNSYLDKVGL VLRVLRALRI LYVMRLARHS361LGLQTLGLTA RRCTREFGLL LLFLCMAIAL FAPLLYVIEN EMADSPEFTS IPACYWWAVI421TMTTVGYGDM VPRSTPGQVV ALSSILGGIL LMAFPVTSIF HTFSRSYLEL KQEQERVMFR481RAQFLIKTKS QLSVSQDSDI LFGSASSDTR DNNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
513 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.