Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Junctin / ASPH Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108795-100
Catalog Number: LS-G108795
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Aspartate beta hydroxylase(ASPH) (NM_032468), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A beta H-J-J | AAH | ASP beta-hydroxylase | BAH | Aspartate beta-hydroxylase | CASQ2BP1 | Cardiac junctin | HAAH | Humbug | JCTN | Junctate | Junctin | ASPH
- Origin Species
- Human
- NCBI GenBank
- NM_004318
- SwissProt
- Q12797
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEDKETKHG GHKNGRKGGL SGTSFFTWFM VIALLGVWTS VAVVWFDLVD YEEVLAKAKD61FRYNLSEVLQ GKLGIYDADG DGDFDVDDAK VLLGLKERST SEPAVPPEEA EPHTEPEEQV121PVEAEPQNIE DEAKEQIQSL LHEMVHAEHV EGEDLQQEDG PTGEPQQEDD EFLMATDVDD181RFETLEPEVS HEETEHSYHV EETVSQDCNQ DMEEMMSEQE NPDSSEPVVE DERLHHDTDD241VTYQVYEEQA VYEPLENEGI EITEVTAPPE DNPVEDSQVI VEEVSIFPVE EQQEVPPDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
299 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.